Checklist for introducing new users to the MPI-CBG Light Microscopy Facility
From BioDIP
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* Send user link to [[New user project questions]]. Ask the user to send all relevant answers to lmf(at)mpi-cbg.de ideally before the first face to face meeting. (It might not make sense for every user to answer every single question). | * Send user link to [[New user project questions]]. Ask the user to send all relevant answers to lmf(at)mpi-cbg.de ideally before the first face to face meeting. (It might not make sense for every user to answer every single question). | ||
* Arrange a personal meeting with the new user and one or more members of the lmf team: discussion about imaging project and agreement on the most suitable system(s) using info from new user questions. | * Arrange a personal meeting with the new user and one or more members of the lmf team: discussion about imaging project and agreement on the most suitable system(s) using info from new user questions. | ||
− | * Point out main contact details: lmf(at)mpi-cbg.de | + | * Point out main contact details: lmf-helpline phone 210-2345 or '''lmf(at)mpi-cbg.de''' (always write to team email, not to single persons!!) . |
* Avoid situations where a new user books a system at short notice and expects to get an introduction all done in 1 or 2 hours with no prior meeting/discussion about their project. | * Avoid situations where a new user books a system at short notice and expects to get an introduction all done in 1 or 2 hours with no prior meeting/discussion about their project. | ||
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* How to book systems and assistance (combined and separately). | * How to book systems and assistance (combined and separately). | ||
* How do blockings work. When and why might the LMF ask users to give up their slot. Make user be aware of and respect the student's system checks! | * How do blockings work. When and why might the LMF ask users to give up their slot. Make user be aware of and respect the student's system checks! | ||
− | * How to use the "[[Report A Problem| | + | * How to use the "[[Report A Problem|Problem]]" function. |
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* AND: "END OF SESSION" means: data saved, sample removed, objectives cleaned, software closed ... system is free for the next user!!! | * AND: "END OF SESSION" means: data saved, sample removed, objectives cleaned, software closed ... system is free for the next user!!! | ||
+ | |||
+ | === Safety and Waste === | ||
+ | |||
+ | * LMF is an S1 area - no S2 GMOs allowed! | ||
+ | * Especially: No eating and drinking in LMF, exception: LMF office | ||
+ | * Laser safety | ||
+ | * Waste: General (no S1, no glass, no sharpies); S1 (only solid, take liquid back to home lab); Glass (yellow bins at systems are S1 glass waste); Sharpies (for sharpies only, are S1) | ||
+ | |||
+ | |||
+ | === General === | ||
* ''' Sign the user into the lmf-user [https://cbrs.mpi-cbg.de/mailinglists/login/| mailing list] ''' | * ''' Sign the user into the lmf-user [https://cbrs.mpi-cbg.de/mailinglists/login/| mailing list] ''' | ||
+ | * '''Explain BioDIP Wiki!''' | ||
+ | |||
+ | * '''If not already done, find an appointment for the user at the system of choice with the responsible assistant and book the slot in the database!''' | ||
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* Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver | * Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver | ||
* Data safety: Point out that the LMF holds no responsibility for data whatsoever! | * Data safety: Point out that the LMF holds no responsibility for data whatsoever! | ||
− | '''Important:''' in order to keep the disk free for imaging - data stored on hard drives will be deleted on an irregular basis without warning. | + | '''Important:''' in order to keep the disk free for imaging - data stored on hard drives older than 1 month will be deleted on an irregular basis without warning. |
* Safety regulations: laser safety, S1 safety, correct use of wastebins. | * Safety regulations: laser safety, S1 safety, correct use of wastebins. | ||
* How to clean objectives. | * How to clean objectives. |
Latest revision as of 11:00, 28 October 2013
Contents |
[edit] First Contact
- Send user link to New user project questions. Ask the user to send all relevant answers to lmf(at)mpi-cbg.de ideally before the first face to face meeting. (It might not make sense for every user to answer every single question).
- Arrange a personal meeting with the new user and one or more members of the lmf team: discussion about imaging project and agreement on the most suitable system(s) using info from new user questions.
- Point out main contact details: lmf-helpline phone 210-2345 or lmf(at)mpi-cbg.de (always write to team email, not to single persons!!) .
- Avoid situations where a new user books a system at short notice and expects to get an introduction all done in 1 or 2 hours with no prior meeting/discussion about their project.
[edit] Administration (no system time needed)
Estimated time: about 30 minutes
[edit] Booking Database
- General structure of database.
- Booking budgets, costs.
- How to book systems and assistance (combined and separately).
- How do blockings work. When and why might the LMF ask users to give up their slot. Make user be aware of and respect the student's system checks!
- How to use the "Problem" function.
[edit] Booking Rules And Responsibilities
- Delete and Cancel: the 24h rule.
- The SIFFY procedure: how to offer and take a free slot.
- At the end of each session, check booking database: last user / not the last user --> what are the responsibilities?
- Consequences for failing to switch off a system: extended booking + 15/30min assistant time
- AND: "END OF SESSION" means: data saved, sample removed, objectives cleaned, software closed ... system is free for the next user!!!
[edit] Safety and Waste
- LMF is an S1 area - no S2 GMOs allowed!
- Especially: No eating and drinking in LMF, exception: LMF office
- Laser safety
- Waste: General (no S1, no glass, no sharpies); S1 (only solid, take liquid back to home lab); Glass (yellow bins at systems are S1 glass waste); Sharpies (for sharpies only, are S1)
[edit] General
- Sign the user into the lmf-user mailing list
- Explain BioDIP Wiki!
- If not already done, find an appointment for the user at the system of choice with the responsible assistant and book the slot in the database!
[edit] Theoretical Background (might be possible without system time)
- Background to the techniques that will be used with the system, eg. contrasting techniques, fluorescence, beampath, pinhole, detector etc.
[edit] On The System
[edit] First Introduction
Usually WITHOUT own sample!!!
Estimated time: system dependent, but usually around 2hours
- Introduction to hardware: how to switch on/off.
- Introduction to software: how to properly acquire an image, z-stack, time series (it might sometimes not be possible to cover all of that in the 1st intro).
- Data handling: explain about original file formats and metadata; advantages/disadvantages of storing data on either hard drive or fileserver
- Data safety: Point out that the LMF holds no responsibility for data whatsoever!
Important: in order to keep the disk free for imaging - data stored on hard drives older than 1 month will be deleted on an irregular basis without warning.
- Safety regulations: laser safety, S1 safety, correct use of wastebins.
- How to clean objectives.
[edit] Follow Up Introduction
With own sample!!!
Estimated time: strongly project dependent, but 1-2 hours are usually a good estimate
- Recap contents of first intro
- Introduce user to things that couldn't be covered in the first session
- Help user to set up the system for their own imaging experiment.
- Stay with user until he/she feels save to use the system independently.
- Invite user to contact the lmf team about questions / problems / changes in their imaging projects at any time.