Biopolis Dresden Imaging Platform

DZNE SD2 Spinning Disk 2 Incubation (Zeiss)

This Spinning disc system comes with a dark microscope stand incubation chamber, with which you can hold a stable temperature and CO2 environment.

Category

Spinning disk

Location

DZNE, LMF DZNE, room 251

Suitable for Specimen
Live samples
Fixed samples
Suitable for Technique
Multiple positions
Large area tile/mosaic scans
Multi wellplate imaging
Microscope Stand

Zeiss Axio Observer.Z1, inverted stand
motorized objective revolver, motorized z-focus

Objectives

5x/0.16 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 12.1 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 17 5x/0.16 Zeiss
Order Number: 420630-9900

20x/0.8 Plan-Apochromat, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 0.55 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 18 20x/0.8 Zeiss
Order Number: 420650-9901

2.5x/0.08 EC Plan-Neofluar, Air, Zeiss

Immersion: Air
Coverslip Thickness: 0.17
Working Distance: 8.8 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 19 2.5x/0.085 Zeiss
Order Number: 420320-9901

40x/0.95 Plan-Apochromat, Air, Zeiss

Immersion: Air
Collar: coverslip correction
Coverslip Thickness: 0.17
Working Distance: 0.25 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 20 40x/0.95 Korr Zeiss
Order Number: 420660-9970

100x/1.4 Plan-Apochromat, Oil, DIC, Zeiss

Immersion: Oil
Coverslip Thickness: 0.17
Working Distance: 0.17 mm
Nomarski Prism: PA 100/1,4 HC II (426949)
Thread: M27
Tube Length: infinity
Objective ID: DZNE 21 100x/1.4 Zeiss
Order Number: 420792-9900

10x/0.3 EC Plan-Neofluar, Air, Ph1, Zeiss

Immersion: Air
Coverslip Thickness: -
Working Distance: 5.2 mm
Thread: M27
Tube Length: infinity
Objective ID: DZNE 22 10x/0.3 Zeiss
Order Number: 420341-9911
Condenser

BF, DIC II, DIC III, Ph1, Ph2, Ph3
NA 0.55, working distance 26 mm, motorized

Fluorescence Filter Cubes

motorized revolver

GFP (set 489038-9901-000)

single cube for Alexa488/GFP, CFP, FITC

  • BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
  • LP 495, dichromatic mirror, longpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss

, set 489038-9901-000

Cy3 (set 43 HE)

single cube for Alexa555/Cy3/RFP

  • BP 605/70 (BP 570-640), excitation filter, bandpass, Zeiss
  • LP 570, dichromatic mirror, longpass, Zeiss
  • BP 550/25 (BP 538-563), emission filter, bandpass, Zeiss

Zeiss, set 43 HE

Texas red (set 49)

single cube for DAPI

  • G 365, excitation filter, bandpass, Zeiss
  • LP 395, dichromatic mirror, longpass, Zeiss
  • BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss

Zeiss, set 49

Cy5 (set 50)

single cube for Cy5

  • BP 690/50 (BP 665-715), excitation filter, bandpass, Zeiss
  • LP 660, dichromatic mirror, longpass, Zeiss
  • BP 640/30 (BP 625-655), emission filter, bandpass, Zeiss

Zeiss, set 50

B-G-R (DAPI/GFP/RFP) (set 62)

triple cube for DAPI/GFP/RFP

  • BP 370/40; BP 474/28; BP 585/35 (BP 350-390; BP 460-488; BP 568-603), excitation filter, bandpass, Zeiss
  • (DM 395+495+610), triple dichromatic mirror, triple bandpass, Zeiss
  • BP 425/46; BP 527/54; LP 615 (BP 402-448; BP 500-554; LP 615), emission filter, triple bandpass, Zeiss

Zeiss, set 62

Additional Filters

DAPI, GFP, Cy3, Cy5

  • BP 450/50 (BP 425-475), emission filter, bandpass, Zeiss
  • BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
  • BP 617/73 (BP 581-654), emission filter, bandpass, Zeiss
  • BP 690/50 (BP 665-715), emission filter, bandpass, Zeiss
Fluorescence Illumination

HXP 120C

Zeiss, Metal halide lamp, 120W, integrated

365, 470, 590, 625 nm

Zeiss, Colibri LED, integrated

405nm

Laser Diode, integrated

488nm

Laser OPSL, integrated

568nm

Laser DPSS

635nm

Laser Diode, integrated
Camera

Zeiss AxioCam MRm, Monochrome CCD camera, dexel size 6.45 µm

port SD port
magnification 1x
bit depth 12 bit
sensor size 8.9 x 6.7 mm
maximum frame size 1388 x 1040 dexel
maximum full chip frame rate 12.86 fps
Serial number 120125512
XY Stage

motorized stage

Märzhäuser Scanningtisch 130x100 STEP with

  • universal holder K (slides, dishes 24-68mm)
  • fixed holder for 4 slides
  • multiwell plate holder
  • heating insert P S1
Hardware Autofocus
Incubation

cage incubator

Zeiss

Feature:

  • CO2

  • Heating

Additional Information
  • System described by manufacturer
  • Laser power values
  • See system check
  • Inventory Number 485
  • SIP Number 3834004994
  • Contact for Service 0176-20463239
  • Service Contract Number PO 4500045803
  • DRESDEN concept ID 2040
  • Date of Installation 26.09.2012
  • End of Warranty 26.09.2014