MZ1 - Zeiss 2photon upright (Zeiss)
Zeiss LSM 780 NLO
Multiphoton Laser Scanning Microscope with a tunable pulsed near-infra-red laser (Chameleon Vision II) for multiphoton excitation. Single/multi-photon point scanning confocal system with two PMTs and one 32-channel GaAsP detector for spectral detection, four non-descanned GaAsP detectors for multi-photon detection, and three transmitted light detectors (two for multi-photon, one for single-photon). Motorized stage for multi-point acquisition.
Category
Laser scanning confocal, Multi photon
Location
MPI-CBG, LMF MPI-CBG, room 038C
Suitable for Specimen
Suitable for Technique
Microscope Stand
Objectives
10x/0.3 EC Plan-Neofluar, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | - |
| Working Distance: | 5.5 mm |
| Thread: | RMS (20.32×0.71 mm) |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 110 10x/0.3 Zeiss |
| Order Number: | 440330-9901 |
10x/0.45 Plan-Apochromat, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 2 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 111 10x/0.45 Zeiss |
| Order Number: | 420640-9900 |
10x/0.5 Clr Plan-Apochromat, Clearing, Zeiss
| Immersion: | Clearing |
| Working Distance: | 3.7 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 331 10x/0.5 Zeiss |
| Order Number: | 421440-9800 |
20x/0.8 Plan-Apochromat, Air, Zeiss
| Immersion: | Air |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.55 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 113 20x/0.8 Zeiss |
| Order Number: | 420650-9901 |
25x/0.8 LD LCI Plan-Apochromat, W / Glyc / Oil, DIC, Zeiss
| Immersion: | W / Glyc / Oil |
| Collar: | immersion correction |
| Coverslip Thickness: | 0-0.17 |
| Working Distance: | 0.57 mm at cover glass 0.17 mm |
| Nomarski Prism: | LD LCI PApo 63/1,20 III (426947) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 246 25x/0.8 Zeiss |
| Order Number: | 420852-9870 |
40x/1 W Plan-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Working Distance: | 2.5 mm |
| Nomarski Prism: | W PA 40/1,0 III (426967-9010) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 114 40x/1.0 Zeiss |
| Order Number: | 421462-9900 |
40x/1.2 C-Apochromat, Water, DIC, Zeiss
| Immersion: | Water |
| Collar: | coverslip correction |
| Coverslip Thickness: | 0.14-0.19 |
| Working Distance: | 0.28 mm at cover glass 0.17 mm |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 115 40x/1.2 Zeiss |
| Order Number: | 421767-9970 |
40x/1.3 C Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.2 mm |
| Nomarski Prism: | C Plan-Apochromat 40x/1,3 III (426965-9000) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 293 40x/1.3 Zeiss |
| Order Number: | 421762-9900 |
63x/1.3 LCI Plan-Neofluar, W / Glyc, DIC, Zeiss
| Immersion: | W / Glyc |
| Collar: | immersion correction |
| Coverslip Thickness: | 0.15-0.19 |
| Working Distance: | 0.17 mm at cover glass 0.17 mm |
| Nomarski Prism: | LCI PN 63/1,30 II HC (426958), LCI PN 63/1,30 III (426959) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 116 63x/1.3 Zeiss |
| Order Number: | 420882-9970 |
| Inventory Number: | 4084160000 |
63x/1.4 Plan-Apochromat, Oil, DIC, Zeiss
| Immersion: | Oil |
| Coverslip Thickness: | 0.17 |
| Working Distance: | 0.19 mm |
| Nomarski Prism: | PA 63/1,4 HC II (426952), PA 63/1,40 III (426957) |
| Thread: | M27 |
| Tube Length: | infinity |
| Objective ID: | MPI-CBG 117 63x/1.4 Zeiss |
| Order Number: | 420782-9900 |
Condenser
Fluorescence Filter Cubes
motorized revolver
GFP (set 38)
single cube for Alexa488/GFP, CFP, FITC
- BP 470/40 (BP 450-490), excitation filter, bandpass, Zeiss
- LP 495, dichromatic mirror, longpass, Zeiss
- BP 525/50 (BP 500-550), emission filter, bandpass, Zeiss
Zeiss, set 38
Cy3.5 (set 38)
DAPI (set 488049-9901-000)
single cube for DAPI
- G 365, excitation filter, bandpass, Zeiss
- LP 395, dichromatic mirror, longpass, Zeiss
- BP 445/50 (BP 420-470), emission filter, bandpass, Zeiss
Zeiss, set 38, set 488049-9901-000
Additional Filters
- LP 690, dichromatic mirror, longpass, Zeiss
- LP 760, dichromatic mirror, longpass, Zeiss
Transmitted Illumination
Lamp (Halogen, 100W)
Fluorescence Illumination
HXP 120C
Zeiss, Metal halide lamp, 120W, manual
405nm
Laser Diode, integrated
561nm
Laser DPSS, integrated
594nm
Laser HeNe, integrated
633nm
Laser HeNe, integrated
458, 488, 514nm multiline
Laser Argon, manual
680-1080 nm
Coherent, Laser Multiphotonen Chameleon, manual
XY Stage
Scan Head
Extra Photo Manipulation
Additional Information