Biopolis Dresden Imaging Platform

BIOTEC/CRTD - Electron Microscopy and Histology Facility

The Electron Microscopy Facility of the CRTD is offering advice and service concerning sample preparation, labeling and imaging of biological specimen in TEM, SEM and CLEM.

The Histology facility of the BIOTEC offers service concerning sample preparation/sectioning of biological material.


Contact

DFG-Center for Regenerative Therapies Dresden
Cluster of Excellence and Biotechnology Center (BIOTEC)
Fetscherstraße 105
01307 Dresden
Germany

e-mail: thomas.kurth[at]crt-dresden.de
phone:
+49 - 351 - 463 82090
web:
BIOTEC/CTRD-EMF webpage


Sample preparation

  • Conventional processing and embedding of cells and tissues into epoxy or methacrylate resins (Epon, Spurrs resin, Lowicryl) including microwave-assisted tissue processing

  • Embedding into gelatin/sucrose followed by plunge freezing for Tokuyasu cryo-sectioning and immunogold labeling

  • High pressure freezing (HPF) and freeze substitution (FS)

  • Variations and combinations of sample preparation methods, such as chemical fixation followed by infiltration in sucrose, plunge freezing, freeze substitution and epon or Lowicryl embedding

  • Ultramicrotomy (room temperature and cryo): semithin sections for light microscopy and ultrathin sections for TEM

  • Negative staining

  • Immunogold labeling of resin or Tokuyasu cryo-sections (postembedding labeling)

  • Preembedding immunolabeling using HRP or Nanogold and silver enhancement

Imaging

  • Data acquisition in transmission electron microscopy (TEM)
  • Correlative light electron microscopy/tomography (CLEM)

In addition to image acquisition, the EM facility provides data storage and image-processing support (registration, 3D reconstruction, image segmentation).

BIOTEC/CRTD EMF


Facility Members


Links