Biopolis Dresden Imaging Platform

MPI-CBG Electron Microscopy Facility

The Electron Microscopy (EM) Facility provides resources, technology and expertise for studying the ultrastructure of cryo-immobilized and chemically fixed biological samples.
Complex projects can be undertaken in collaboration with the facility.

We are also developing technologies in electron tomography, correlative light/electron microscopy (CLEM) and scanning electron microscopy (SEM).


Contact

Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG)
Electron Microscopy Facility
Pfotenhauerstr. 108
01307 Dresden
Germany

e-mail: em[at]mpi-cbg.de
phone:
+49 - 351 - 210 17
web:
MPI-CBG EMF webpage

 


Services

Sample preparation

  • Sample preparation for cryo-immobilization (High-pressure freezing, Plunge freezing), or chemical fixation, including microwave processing
  • Sectioning: ultrathin, semi-thin, serial sections, cryo-sections (Tokuyasu)
  • Positive contrasting (pre- or post-embedding) and negative staining
  • Immuno-labelling/staining (as pre-embedding or labeling on plastic- and cryo-sections)

Imaging

  • Data acquisition in transmission electron microscopy (TEM), cryoTEM or SEM
  • Electron tomography (3D imaging)
  • Correlative light electron microscopy/tomography (CLEM)
  • Serial block-face scanning electron microscopy (SBF-SEM)

In addition to image acquisition, the EM facility provides data storage and image-processing support (registration, 3D reconstruction, image segmentation).


Facility Members


Links