BasicsCoursePhDprog-PeterEvennetMay2011-observed timing

PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011

Setup -Fri 13 May

Setup - all lmf staff move stuff to galleria Equipment needed

• Microscopes
• Optical bench
• Spinning disk gadget
• Polarazing sheets
• Diffraction Grids
• Fluorescent samples
• Fluorescent Beads (Prepare slides)
• First day Handout for students
• Light torches

DAY ONE (16th May)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	09:30	FINAL PREP	Test everything works	LMF TEAM
9:30	10:15	INTRODUCTION	COURSE INTRO WHO is WHO? Round table	LMF TEAM
10:15	10:55	Session 1 Theory	PRINCIPLES OF LIGHT MICROSCOPY Historical aspects Very basic components and principles ( incl. Refraction-basics 1st part)	PETER EVENNETT
10:55	11:15	BREAK	BREAK	BREAK
11:20	12:45	SESSION 1 Theory	Refraction-basics 2nd part (Coin-in-cup-demo) Introduction to lenses (Lens demo fun) Lens aberrations (see colour fringes of lighting image with simple lens) Magnification Resolution, Numerical aperture	PETER EVENNETT
12:45	13:45	LUNCH	LUNCH	LUNCH
13:45	14:45	SESSION 1 Practice 15min (!!!) Rotation	Milky Glass Block demo Show and discuss microscope parts (upright and inverted) NA measurements	DAN JAN & BRITTA SILKE
14:45	15:30	SESSION 2 Theory	Microscope illumination Conjugate planes	PETER EVENNETT
15:30	15:45	BREAK	BREAK	BREAK
15:45	16:50	SESSION 2 Practice (30min Rotation)	conjugate planes on Peters microscope conjugate planes on optical bench	PETER EVENNETT JAN
16:50	17:15	SESSION 3 Theory	Koehler Illumination (Microscope alignment)	PETER EVENNETT
17:15	18:30	SESSION 3 Practice	Koehler illumination (Microscope alignment)	LMF TEAM

DAY TWO (May 17th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	10:00	Recap	day 1	LMF TEAM
10:00	10:20	SESSION 1 Theory & Practice	Depth of Field Depth of Focus	PETER EVENNETT LMF TEAM
10:20	11:15	SESSION 2 Theory & Demo	Diffraction Diffraction slide fun	PETER EVENNETT
11:15	11:30	BREAK	COFFEE BREAK	Note: have break earlier next time, right after diffraction-string-show (should be shortly before 11am) then do all the explanation of Abbe's Diffraction Exp after break
11:30	12:50	DEMONSTRATION	ABBE'S DIFFRACTION EXPERIMENT Peter's Video (50min) Dan's setup demonstration	PETER EVENNETT DAN
12:50	13:50	BREAK	LUNCH
13:50	14:05		spontaneous discussion :-)
14:05	14:55	SESSION 3 Theory	Lens aberrations and corrections Objectives	PETER EVENNETT
14:55	15:10	BREAK	COFFEE BREAK
15:10	15:55	SESSION 4 Theory	Dark Field microscopy	PETER EVENNETT
15:55	16:30	SESSION 4 Practice	Dark Field microscopy	LMF TEAM
16:30	16:50	SESSION 5 Theory	Basics of Phase Contrast microscopy	PETER EVENNETT
16:50	17:30	SESSION 5 Practice	Phase Contrast microscopy	LMF TEAM
17:30	:	SESSION 5 Theory	Details of Phase Contrast microscopy	PETER EVENNETT

DAY THREE (May 18th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	10:00	Recap	day 2	LMF TEAM
10:00	10:20	SESSION 1 Theory	Objective reading	PETER EVENNETT LMF TEAM
10:20	10:35	BREAK	BREAK	BREAK
10:40	12:10	SESSION 1 Practice Group rotation (30min. each!)	Cover glasses and sample mounting Objective reading Objective cleaning	Dan Britta, Silke Jan
12:10	13:00	SESSION 2 Theory	Polarized light microscopy	PETER EVENNETT
13:00	14:00	BREAK	LUNCH
14:00	14:40	SESSION 2 Practice	Polarized light microscopy	LMF TEAM
14:40	15:05	SESSION 3 Theory	Differential Interference Contrast (DIC)	PETER EVENNETT
15:05	15:40	SESSION 3 Practice	Differential Interference Contrast (DIC)	LMF TEAM
15:40	16:00	BREAK	BREAK	BREAK
16:00	16:30	Question round	Questions to Peter Evennett	PETER EVENNETT LMF TEAM
16:30	17:40	SESSION 4 Theory & Demo	What is a pixel Analog to Digital conversion Nuyquist-Shannon	Dan
17:40	18:00	SESSION 3 Practice	PIXELS Sesame seeds, sunflower seeds, nudels, gumibears and a checker board	LMF TEAM

DAY FOUR (May 19th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)	DEMO Activities	DEMO materials
9:00	10:00	Recap	day 3	LMF TEAM
10:00	10:45	SESSION 1 Theory	Fundamental concepts of fluorescence	DAN	-	-
10:45	11:00	BREAK	BREAK	BREAK	BREAK	BREAK
11:00	12:15	SESSION 2 Theory & Practice	Fluorescence Microscopy	SILKE LMFTEAM	• Light sources • Lamp houses • Spectra • Filters • Filter cubes • Filter spectral charts of each microscope (laminated)	• Spare lamp houses • Lamps • Ocean optics equipment • Four (4) different Filter-sets (cubes), with the appropriate descriptions • diagramms for drawing filter/fluorophore spectra
12:15	13:00	SESSION 2 Practice	Fluorescence imaging at microscopes	LMF TEAM	-	• Fluorescent labeled samples
13:00	14:00	BREAK	LUNCH	LUNCH	LUNCH	LUNCH
14:00	15:45	SESSION 3 Theory & Practice	Imaging with CCD and Quantitative Imaging	LMF TEAM Britta/Dan	• CCD • Cameras	• CCD chip • RGB Slider Spot Camera setup
15:45	16:00	BREAK	BREAK
16:00	18:00	SESSION 4 Practice	Imaging with CCD cameras using LMF systems	LMF TEAM	• Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast	• Microscopes: DVcore(Dan, 3 students) pDV (Silke, 2 students) Inverted CCD (Pete, 2 students) N-Storm (Britta, 2 students) self-built-syst (Jan, 3 students)) • Fluorescence Sample slides (Kidney / Cells)
19:00	open end	Dinner	with Peter	LMF TEAM

DAY FIVE (May 20th)

FROM	TO	ACTIVITY	TOPICS	Responsible person(s)
9:00	10:00	Recap	day 4	LMF TEAM
10:00	11:10	SESSION 1 Theory & Demo	Optical sectioning methods Pros&Cons	LMF TEAM Dan / Jan
11:10	11:25	BREAK	BREAK	BREAK
11:25	13:00	SESSION 1 Theory & Demo	Optical sectioning methods Pros&Cons	LMF TEAM Dan / Jan
13:00	14:00	BREAK	LUNCH
14:00	15:00	SESSION 2 Small Groups Discussion	What techniques might be good for me (Own Projects)	LMF TEAM Dan/Jan/Silke/Britta/Pete?
15:00	15:15	BREAK	BREAK	BREAK
15:15	16:00	SESSION 3	Course evaluation	LMF TEAM