View source for CZ4 - Zeiss LSM DuoScan

{{Equipment
|system-number=MPI-CZ4
|system-name=Zeiss LSM DuoScan
|type=Not listed
|category=Laser Scanning Confocal
|facility=MPI-CBG LMF
|building=MPI-CBG
|room=038A
|Facility-logo=MPI-CBG-Lmf-logo-big.png
|Institute-logo=Logo MPI-CBG.jpg
|description=Single photon point scanning [[Zeiss LSM 510]] [[confocal]] system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition. AIM SYSTEM 2435000381
|applications=spectral imaging, routine dye combinations, [[tile scan]], multi position scan
|image=CZ4 - Zeiss LSM DuoScan.jpg
|stand-name=Zeiss - Axio Observer
|stand=Inverted
|microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
|obj1=Zeiss Plan-Apochromat 10x 0.45
|obj2=Zeiss Plan-Apochromat 20x 0.8
|obj3=Zeiss C-Apochromat 40x 1.2 W
|obj4=Zeiss Plan-Apochromat 63x 1.4 Oil
|obj5=Zeiss LCI-PLAN Neofluar 25x0.8 Oil/Glyc/Water DIC 0-0.17
|obj6=Zeiss EC Plan-Neofluar 40x 1.3 Oil
|ill1=Transmitted Light (Halogen)
|ill2=Fluorescence (Metal Halide, X-Cite, 120W)
|ill3=Laser Diode 405 nm
|ill4=Laser Argon Multiline 458, 477, 488, 514 nm
|ill5=Laser DPSS 561 nm
|ill6=Laser HeNe 633 nm
|ill7=Laser DPSS 488 nm
|detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector
|reflectors=1. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488049-0000-000  Filter Set 49]. G365 * FT395 *   BP445/50 <br>2. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488013-0000-000  Filter Set 13]. BP 470/20 * FT493 * BP505-530 <br>3. [https://www.micro-shop.zeiss.com/us/us_en/spektral-info.php?i=488015-0000-000 Filter Set 15]. BP546/12 * FT580 * LP590.
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
|software=-
|incubation=Stage incubator (T) + objective heater
|link1=[http://spreadsheets.google.com/pub?key=pFACvabR4mSl8mKAtkf_cZg LPM]
|link2=[https://spreadsheets.google.com/pub?key=pMZJXCgd5HNtGjGuWC1GTCQ scan-head configuration]
|inv=AIM SYSTEM 2435000381    10.1.145.12
|facility_image=logo-lmf.jpg
|objectives=10x/0.3<br>20x/0.8 Apo<br>40x/1.2 W Apo<br>63x/1.4 Oil Apo<br>100x/1.45 Oil<br>60x/1.2 W Apo
|illumination=Halogen<br>Metal-Halide<br>405 nm Diode laser<br>458, 477, 488, 514 nm Argon laser<br>561 nm DPSS laser<br>633 nm HeNe laser
}}