H1 - DeltaVision OMX - pDV

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|room=036
 
|room=036
 
|Facility-logo=MPI-CBG LMF.jpg
 
|Facility-logo=MPI-CBG LMF.jpg
|description=Structured Illumination Superresolution and very fast widefield. 4x sCMOS, 4x laser illumination widefield or structured 3D illumination. Also has its own personal DeltaVision widefield fluorescence microscope system with a stage that is registered with the OMX stage, for locating samples on the slide.
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|description=Applied Precision Inc Deltavision OMX v4 BLAZE: Live cell and fixed sample Structured Illumination Superresolution and very fast widefield. 4x sCMOS cameras, 405, 488, 568, 640 laser illumination widefield or structured 3D illumination. Also has its own personal DeltaVision widefield fluorescence microscope system with a stage that is registered with the OMX stage, for locating samples on the slide.
 
|applications=super resolution of fixed and living samples down to 120 nm, and very fast widefield imaging.
 
|applications=super resolution of fixed and living samples down to 120 nm, and very fast widefield imaging.
 
|image=H1-OMX.jpg
 
|image=H1-OMX.jpg

Revision as of 17:42, 11 January 2012

Suitable for super resolution of fixed and living samples down to 120 nm, and very fast widefield imaging..
H1-OMX.jpg


Directions

[[image:|150px|text-bottom|right]]

MPI-CBG LMF.jpgMPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Applied Precision - OMX stand, Inverted stand, nano precise XYZ stage, fast z peizo, , fast structured illumination OMX Blaze, fluorescence, transmitted light, DIC, plus IX71 for the pDV.
objectives
Olympus UPlanSApo 100x 1.4 Oil
Olympus UPlanSApo 10x 0.4
Olympus PlanApo N 60x 1.42 Oil UIS2 inf/0.17/FN26.5 WD 0.15 mm
illumination
Transmitted Light (LED)
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection 4x sCMOS cooled to 3oC. Simultaneous or sequential imaging.
reflectors "standard" set for fixed samples (also GFP, and some red proteins), OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set.
features fast 4 colour widefield. Super resolution by structured illumination in 3D, down to approx 120 nm resolution (2x the resolution of conventional light microscopy)
software API softworx and OMX software for driving the scope and super resolution reconstruction.

OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32"API softworx and OMX software for driving the scope and super resolution reconstruction. OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32" cannot be used as a page name in this wiki.

incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZG040809900



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