H1 - DeltaVision OMX - pDV

From BioDIP
(Difference between revisions)
Jump to: navigation, search
Line 8: Line 8:
 
|room=036
 
|room=036
 
|Facility-logo=MPI-CBG LMF.jpg
 
|Facility-logo=MPI-CBG LMF.jpg
|description=Structured Illumination Superresolution and fast widefield. 4x EM-CCD cameras (sCMOS soon), 4x laser illumination widefield or structured 3D illumination. Also has its own personal DeltaVision widefield fluorescence microscope system with a stage that is registered with the OMX stage, for locating samples on the slide.
+
|description=Structured Illumination Superresolution and very fast widefield. 4x sCMOS, 4x laser illumination widefield or structured 3D illumination. Also has its own personal DeltaVision widefield fluorescence microscope system with a stage that is registered with the OMX stage, for locating samples on the slide.
|applications=super resolution down to 120 nm, and fast widefield imaging.
+
|applications=super resolution of fixed and living samples down to 120 nm, and very fast widefield imaging.
 
|image=H1-OMX.jpg
 
|image=H1-OMX.jpg
 
|stand-name=Applied Precision - OMX stand
 
|stand-name=Applied Precision - OMX stand
 
|stand=Inverted
 
|stand=Inverted
|microscope=nano precise XYZ stage, fluorescence, transmitted light, DIC,  
+
|microscope=nano precise XYZ stage, fast z peizo, , fast structured illumination OMX Blaze, fluorescence, transmitted light, DIC, plus IX71 for the pDV.
plus IX71 for the pDV.  
+
 
|obj1=Olympus UPlanSApo 100x 1.4 Oil
 
|obj1=Olympus UPlanSApo 100x 1.4 Oil
 
|obj2=Olympus UPlanSApo 10x 0.4
 
|obj2=Olympus UPlanSApo 10x 0.4
Line 23: Line 22:
 
|ill4=Laser DPSS 561 nm
 
|ill4=Laser DPSS 561 nm
 
|ill5=Laser DPSS 640 nm
 
|ill5=Laser DPSS 640 nm
|detection=4x EM-CCD (soon to be sCMOS instead). Simultaneous or sequential imaging.
+
|detection=4x sCMOS cooled to 3oC. Simultaneous or sequential imaging.
|reflectors=standard set, OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set.  
+
|reflectors="standard" set for fixed samples (also GFP, and some red proteins), OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set.
|features=fast 4 colour widefield. Super resolution by structured illumination in 3D, down to just over 100 nm resolution (2x the resolution of conventional light microscopy)
+
|features=fast 4 colour widefield. Super resolution by structured illumination in 3D, down to approx 120 nm resolution (2x the resolution of conventional light microscopy)
 
|software=API softworx and OMX software for driving the scope and super resolution reconstruction.
 
|software=API softworx and OMX software for driving the scope and super resolution reconstruction.
 
OMX SI reconstruction linux box: centos 4,  10.1.145.31
 
OMX SI reconstruction linux box: centos 4,  10.1.145.31

Revision as of 11:19, 11 January 2012

Suitable for super resolution of fixed and living samples down to 120 nm, and very fast widefield imaging..
H1-OMX.jpg


Directions

[[image:|150px|text-bottom|right]]

MPI-CBG LMF.jpgMPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Applied Precision - OMX stand, Inverted stand, nano precise XYZ stage, fast z peizo, , fast structured illumination OMX Blaze, fluorescence, transmitted light, DIC, plus IX71 for the pDV.
objectives
Olympus UPlanSApo 100x 1.4 Oil
Olympus UPlanSApo 10x 0.4
Olympus PlanApo N 60x 1.42 Oil UIS2 inf/0.17/FN26.5 WD 0.15 mm
illumination
Transmitted Light (LED)
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
detection 4x sCMOS cooled to 3oC. Simultaneous or sequential imaging.
reflectors "standard" set for fixed samples (also GFP, and some red proteins), OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set.
features fast 4 colour widefield. Super resolution by structured illumination in 3D, down to approx 120 nm resolution (2x the resolution of conventional light microscopy)
software API softworx and OMX software for driving the scope and super resolution reconstruction.

OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32"API softworx and OMX software for driving the scope and super resolution reconstruction. OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32" cannot be used as a page name in this wiki.

incubation Stage incubator (T) + objective heater
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZG040809900



Personal tools