H2 - Nikon STORM - TIRF
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(Difference between revisions)
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|Facility-logo=MPI-CBG-Lmf-logo-big.png | |Facility-logo=MPI-CBG-Lmf-logo-big.png | ||
|Institute-logo=Logo MPI-CBG.jpg | |Institute-logo=Logo MPI-CBG.jpg | ||
− | |description=Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW) | + | |description=Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers; |
|applications=N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging | |applications=N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging | ||
|image=H2 - Nikon STORM - TIRF.jpg | |image=H2 - Nikon STORM - TIRF.jpg | ||
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|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
|ill2=Fluorescence (LED) | |ill2=Fluorescence (LED) | ||
− | |ill3=Laser | + | |ill3=Laser DPSS 488 nm |
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
|ill5=Laser DPSS 640 nm | |ill5=Laser DPSS 640 nm | ||
|detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | |detection=at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um<br>at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | ||
− | |reflectors=Microscope stand:<br>reflector revolver FL1 (lower floor):<br>position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 4: analyzer 1, 5: analyzer 2, 6: empty<br> reflector revolver FL2 (upper floor):<br> position 1: TIRF1 cube (quad DC only: zt405/488/561/640rpc), 2: TIRFQ cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28 , 3: LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60 | + | |reflectors=Microscope stand:<br>reflector revolver FL1 (lower floor):<br>position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 4: analyzer 1, 5: analyzer 2, 6: empty<br> reflector revolver FL2 (upper floor):<br> position 1: TIRF1 cube (quad DC only: zt405/488/561/640rpc), 2: TIRFQ cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty <br>Sutter filter wheel:<br>position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 <br>Hamamatsu W-View image splitter:<br>for bypass mode: silver mirror<br>for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 |
|features=- | |features=- | ||
|software=NIS Elements with N-STORM module | |software=NIS Elements with N-STORM module |
Revision as of 18:04, 7 January 2015
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens"motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br />motorized TIRF-condenser with additional manual collimating lens" cannot be used as a page name in this wiki. | ||
objectives | |||
illumination | |||
detection | at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD with cylindrical lens; dexel size of camera chip: 16um at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um | ||
reflectors | Microscope stand: reflector revolver FL1 (lower floor): position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 4: analyzer 1, 5: analyzer 2, 6: empty reflector revolver FL2 (upper floor): position 1: TIRF1 cube (quad DC only: zt405/488/561/640rpc), 2: TIRFQ cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty Sutter filter wheel: position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 Hamamatsu W-View image splitter: for bypass mode: silver mirror for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40 | ||
features | - | ||
software | NIS Elements with N-STORM module | ||
incubation | Cage incubator (T+CO2) | ||
links |
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inv.nr. | 0 10.1.32.156 |