H3 Andor STORM Spinning disc
From BioDIP
(Difference between revisions)
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|ill5=Laser DPSS 561 nm | |ill5=Laser DPSS 561 nm | ||
|ill6=Laser DPSS 640 nm | |ill6=Laser DPSS 640 nm | ||
− | |detection=Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: <br>dichromatic mirror:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>camera: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um<br> | + | |detection=Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: <br>dichromatic mirror:<br>[http://www.semrock.com/FilterDetails.aspx?id=Di01-T405/488/568/647-13x15x0.5 Quad band T-405/488/568/647] <br>physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar)<br>camera: <br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x<br> |
image pixel sizes:<br> | image pixel sizes:<br> | ||
with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.), 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.), 0.0698um/px (1.5x opt.) | with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.), 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.), 0.0698um/px (1.5x opt.) |
Revision as of 14:17, 18 March 2020
[[image:|250px]] |
Directions
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: dichromatic mirror: Quad band T-405/488/568/647 physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x image pixel sizes: | ||
reflectors | Rotr filter wheel: position 0: 520/15, 1: 628/40, 2: 450/50 (447/60 until 2018-04-09), 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73 | ||
features | FRAPPA coupled in via backport | ||
software | [[software::iQ 2.9 + iQ 3.4.1 (both with Python) iQ3 user guide connections: COM1 - ????; COM2xx - lasers; COM3 - ???; COM4 - ???; COM5 - ???; COM6 - ???; AOTF - ALC401/501/601; DD A06/16]] | ||
incubation | Stage incubator (T+CO2) | ||
links |
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inv.nr. |