H3 Andor STORM Spinning disc

Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution.

[[image:|250px]]

Directions

MPI-CBG LMF (MPI-CBG, 340)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Nikon TiE, Inverted stand, motorized with PFS (hardware autofocus), 1.5 x Optovar, MCL Piezo 100um travel range, motorized XY stage
objectives	Nikon Plan-Apochromat 10x 0.45 DIC Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC Nikon Plan-Apochromat 60x 1.2 W Nikon Apo 100x/1.49 Oil DIC 0.13-0.20
illumination	Transmitted Light (LED) Fluorescence (Metal Halide) Laser Diode 405 nm Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm
detection	[[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm), manual: dichromatic mirror: Quad band T-405/488/568/647 physical pinhole radius: 25um, pinhole spacing: 250um; back-projected pinhole size: 0.4um, back-projected pinhole spacing: 4um (with 60x objective, 1x optovar) camera: Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; magnification of camera projection lens: 1.52x image pixel sizes: with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.), 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.), 0.0698um/px (1.5x opt.)]]
reflectors	Rotr filter wheel: position 0: 520/15, 1: 628/40, 2: 450/50 (447/60 until 2018-04-09), 3: 607/36, 4: dual 512/630, 5: LP 664 RU, 6: 525/30, 7: 716/40, 8: LP 488 razor edge, 9: 617/73
features	FRAPPA coupled in via backport
software	[[software::iQ 2.9 + iQ 3.4.1 (both with Python) iQ3 user guide connections: COM1 - ????; COM2xx - lasers; COM3 - ???; COM4 - ???; COM5 - ???; COM6 - ???; AOTF - ALC401/501/601; DD A06/16]]
incubation	Stage incubator (T+CO2)
links	[ manual] [ laser power values] [- beam path] [ advanced system check]
inv.nr.

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