LSM 780/FLIM, inverse, CRTD

Suitable for the detection of multiple fluorescent dyes (but no DAPI), FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector.

Directions

Imaging@BIOTEC/CRTD (CRTD, -1.124)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope	Zeiss - Axio Observer, Inverted stand, -
objectives	Zeiss Plan-Apochromat 10x 0.45 Zeiss Plan-Apochromat 20x 0.8 Zeiss C-Apochromat 40x 1.2 W Zeiss alpha Plan-Apochromat 63x 1.46 Oil
illumination	Laser Diode 440 nm (pulsed) Laser Diode 475 nm (pulsed) Laser Argon Multiline 458, 488, 514 nm Laser DPSS 561 nm Laser HeNe 633 nm Fluorescence (Metal Halide, HXP, 120W) Transmitted Light (Halogen)
detection	32 PMT channels (GaAsP) 2 PMTs transmission detector
reflectors	Zeiss set 49: DAPI Zeiss set 38: GFP Zeiss set 47: CFP Zeiss set 46: YFP Zeiss set 43: Cy3
features	spectral scan, FLIM dual channel unit from B&H
software	ZEN black 2011
incubation	Cage incubator (T+CO2+Humidity)
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.	84844

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