View source for MPI-CZ2 - Zeiss LSM UV - startup & shutdown

== Startup ==

=== Control Panel ===
[[image:remote_control_UV.png|250x125px]]

Switch on Laser Cooling Unit FIRST, then the main Remote Control. 

=== Software Initiation ===

[[image:icon.png|100x50px]] [[image:switchboard.png|300x150px]]
# Start the software
# Make sure "Scan New Images" is clicked
# Then press "Start Expert Mode"
=== Basic Acquisition Setup ===
[[image:software_menu.png|500x250px]]

This is the main software window. From here you can access all needed control windows (bottom row buttons)

[[image:laser_control.png|200x100px]] [[image:microscope_control.png|500x250px]] [[image:config_control.png|500x250px]] [[image:scan-mode.png|500x250px]] [[image:scan_control.png|500x250px]]
# Laser Control <br> Switch on the lasers you need. <br> Remember: for Argon laser use tube current of around 6A
# Microscope Control <br> Here you can make changes to the microscope stand itself. <br> Note: most of these functions you can also directly access via button on the microscope stand.
# Conficuration Control <br> Here you can set up your beam path configuration. <br> Use "Single Track" for simultaneous, "Multi Track" for sequential imaging <br> To set up everything properly, you need to know the excitation and emission spectra of each of your dyes. Please use the provided fluorescence database links
# Scan Control
#* Mode <br> Adjust your scan settings, such as pixel size, scan speed, averaging, zoom,...
#* Channels <br> Adjust pinhole size, detector gain, amplifier offset, and laser power for each channel separately

=== Observation Modes ===
[[image:switch.png|150x75px]]

Use the buttons on the right side of the main software window to switch between visual (VIS; observation by eye) and Laser Scanning Mode (LSM; observation over displays)

== Shutdown ==

'''Before you do anything, please check the LMF booking database at the END(!) of your imaging session to see whether you are the last user or not!!!'''

=== If you are not the last user... ===
[[image:software_menu.png|400x200px]]

# Close the main software window. In order to do this, either choose "File" > "Exit", or click the "x" in the top right corner. You may be prompted to save open image data.
# [[image:switchboard.png|200x100px]] [[image:laser warning.png|150x75px]]
# Back in the "Switchboard", click "Exit". In case lasers are running, a window will pop up asking you what you want to do with these lasers. Since there is another user coming, you leave the lasers on and click ok.
# Upload your data to your file server.
# Log off the windows account via "Start" > "Log off...".

=== If you are the last user... ===

[[image:laser_control.png|200x100px]] [[image:software_menu.png|400x200px]]

# Go to the laser control and switch off all running laser!
# Then close the main software window. In order to do this, either choose "File" > "Exit", or click the "x" in the top right corner. You may be prompted to save open image data.
#[[image:switchboard.png|200x100px]]  
# Back in the "Switchboard", click "Exit". A window will pop up warning you that the lasers still need time to cool down. Click ok.
# Upload your data to your file server.
# Shut down windows via "Start" > "Shut down...".

'''Wait 10 minutes before you proceed! This time is needed to cool down the lasers after you switched them off in the software!!'''
Then..

[[image:remote_control_UV.png|200x100px]]
# Switch off the Remote Control (small box).
# Last, switch off the control box for the laser cooling unit (big box)


[[category:hardware]]
[[category:MPI-CZ2 manuals]]
[[category:CarlZeissLSM510]]