MPI-MZ1 - Zeiss 2photon upright - startup & shutdown

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== How to start the Olympus FV1000 ==
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== How to switch off the Zeiss 2photon upright ==
  
 
=== Hardware ===
 
=== Hardware ===
[[image:mpi09_561.jpg|200x100px]]
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[[image:mpi09_argon-633.jpg|200x100px]]
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[[image:mpi09_powermanager.jpg|200x100px]]
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[[image:mpi09_stagecontroller.jpg|200x100px]]
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# Switch on lasers you need:
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#* 633nm laser: turn key
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#* Argon laser: switch button, then turn the key
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#* 561nm laser: press the green button
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# Switch on the motorised stage
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# Switch on the rest of the system using the power manager below the monitors. Think about what components you really need (otherwise leave them switched off):
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#* LD405 ... 405nm imaging laser
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#* 405nm cutter ... bleaching/ ablation laser
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=== Software Initiation===
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[[image:mpi09_sw-icon.jpg|200x100px]] [[image:mpi09_sw-login.jpg|200x100px]]
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# Start software and log in as lmf user.
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=== Basic Acquisition Setup ===
 
=== Basic Acquisition Setup ===
[[image:mpi09_sw-dyelist.jpg|200x100px]] [[image:mpi09_sw-acquisition.jpg|200x100px]] [[image:mpi09_sw-explorer.jpg|200x100px]]
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# If applicable, use the Olympus Dye list to help you set up the beam path settings.
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# Set up the aquisition parameters according to your sample and needs. Once you have an image, use the HV and offset function to adjust for saturation and background.
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# You might want to reuse parameters from an old image using the Explorer. Look for your image data, right mouse click on it and choose “Reload aquisition parameters”
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=== Basic Troubleshooting ===
 
=== Basic Troubleshooting ===
[[image:mpi09_trouble-shutter.jpg|200x100px]] [[image:mpi09_trouble-sim.jpg|200x100px]]
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* PROBLEM: No light at the object plane!
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** CHECK: excitation shutter of turret open??
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* PROBLEM: No 405nm imaging laser at the object plane!
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** CHECK: SIM function disabled??
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[[category:hardware]]
 
[[category:hardware]]
 
[[category:MPI-MZ1 manuals]]
 
[[category:MPI-MZ1 manuals]]

Revision as of 16:39, 7 April 2010

Contents

How to switch off the Zeiss 2photon upright

Hardware

Basic Acquisition Setup

Basic Troubleshooting

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