MPI-W2 - DeltaVision Core - Startup & shutdown

From BioDIP
(Difference between revisions)
Jump to: navigation, search
(Hardware: updated for new pdu)
 
Line 9: Line 9:
 
Open the plastic door on the computer cabinet, by pressing in the plastic catch button on the little handle.  
 
Open the plastic door on the computer cabinet, by pressing in the plastic catch button on the little handle.  
  
#Switch on the Small Green rectangular power button
+
#Turn on the Black power switch located on the lowest rack - the power distribution unit. 
## this turns in the instrument/computer power strip and the camera
+
## this turns in the instrument/computer power, the camera and the SSI light source.
 
#Switch on the Linux computer with the biggest button (might need to press 2x)
 
#Switch on the Linux computer with the biggest button (might need to press 2x)
#Switch the instrument controller Windows PC with the nice circular metal button.  
+
#Switch the instrument controller Windows PC (ICMIC) with the nice circular metal button.  
 
#The weather station temperature controller (top left of the system) should be left on at all times, usually at 25oC, unless someone wants to use it a 37oC.  
 
#The weather station temperature controller (top left of the system) should be left on at all times, usually at 25oC, unless someone wants to use it a 37oC.  
# Heated 5% CO2 flow can be turned on by turning on only the power switch of the CO2 mixer - grey box on the shelf, and the flow controller on - small black box on top of the weather station. Don't change the position of any of the valves or regulators. You should see bubbles in the humidifier (which should have enough water in it) and flow in the flow meter (6 ml per min). You can match the temperature of the gas to the temperature of the weather station heater controller by changing the setting on the gas mixer.  
+
##this is not connected to the power distribution unit, but plugged straight into the wall socket.
 
+
# Heated 5% CO2 flow can be turned on by turning on the power switch of the CO2 mixer - grey box on the shelf, and the flow controller on - small black box on top of the weather station. Don't change the position of any of the valves or regulators. You should see bubbles in the humidifier (which should have enough water in it, up to the line but no more) and flow in the flow meter (more than 6 ml per min). You can match the temperature of the gas to the temperature of the weather station heater controller by changing the setting on the gas mixer.
  
 
=== Software Initiation ===
 
=== Software Initiation ===

Latest revision as of 13:43, 12 January 2012

Contents

[edit] Startup

[edit] Hardware


Open the plastic door on the computer cabinet, by pressing in the plastic catch button on the little handle.

  1. Turn on the Black power switch located on the lowest rack - the power distribution unit.
    1. this turns in the instrument/computer power, the camera and the SSI light source.
  2. Switch on the Linux computer with the biggest button (might need to press 2x)
  3. Switch the instrument controller Windows PC (ICMIC) with the nice circular metal button.
  4. The weather station temperature controller (top left of the system) should be left on at all times, usually at 25oC, unless someone wants to use it a 37oC.
    1. this is not connected to the power distribution unit, but plugged straight into the wall socket.
  5. Heated 5% CO2 flow can be turned on by turning on the power switch of the CO2 mixer - grey box on the shelf, and the flow controller on - small black box on top of the weather station. Don't change the position of any of the valves or regulators. You should see bubbles in the humidifier (which should have enough water in it, up to the line but no more) and flow in the flow meter (more than 6 ml per min). You can match the temperature of the gas to the temperature of the weather station heater controller by changing the setting on the gas mixer.

[edit] Software Initiation

  1. Log in to the Linux machine - usual LMF username and password (no spaces here... it's Linux)
  2. Start the software by double clicking the SoftWorx icon on the desktop.
  3. In the main window, do File - Acquire (Resolve 3D). This will start up the instrument controller, which will ask you to lower the objective all the way to the bottom position so it can initialize the stage motors. Make sure the 10x objective or another other objective is out of the way before you click OK so the stage does not hit anything as it does it's little dance. In a couple of minutes it will be done.
  4. There is no longer a Xenon lamp to switch on, so the lamp icon is gone. Now the new SSI light source turns itself on and off as needed.

[edit] Observation Modes

In the bottom front of the microscope stand there is a rotatable switch to send light to the eyepieces or the CCD camera. It is manual, the software can not move it. Be gentle with it, these break easily.

[edit] Basic Acquisition Setup

This is the Resolve3D window. From here you can access all needed control windows (bottom row buttons)

  1. Choose the right objective lens.
    Onlt the 10x air lens is kept on the stand. The other objective are stored on the bottom drawer.
    Choose the lens you need, carefully remove it from its box and screw in into the one free position in the objective revolver. You now MUST tell the software what lend you hare using, as it can not sense it. You also must tell the software if the auxiliary magnification (optovar) on the right side of the stand - hidden under the weather station box - is in or out. In is 1x (Aux Mag box unchecked), out is 1.6x (Aux Mag box checked).
    Click the INFO button to get the gadget that calculates what immersion oil you need to use, depending on temperature, mounting medium, coverglass thickness etc.
  2. Filter position viewer
    Here you can see the positions the EX (fluo excitation), EM (fluo emission filter in front of CCD camera), EP (emission filter in front of eyepieces) and %T ND filter (for fluo and transmitted light) positions are
    1. Note: You must select the correct dichomatic mirror for your samples, by rotating the filter cube revolver under the objective revoler.
      1. You must go to Settings - Misc - and change the filter wheemns settings for all three wheels, EXcitation, EMission and EyePeice to the correct setting to match the dichromatic miirror you are using, Standard, live or Alexa - as per the chart stuck to the left side of the weather station env. control box.
    2. Note: Changing the manual EP wheel also chooses the correct EX and EM filters. But not the other way around. Changing EX in the software also selects the correct EM filter position, but not the EP filter wheel position - it cant do that - its not motorised. It can only sense its position, but not move itself. Thats a feature not a bug.
    3. Filter specifications are shown in this pdf: File:Filter Positions Hard Coat File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf


[edit] Shutdown

Before you do anything, please check the LMF booking database at the END(!) of your imaging session to see whether you are the last user or not!!!

[edit] If you are not the last user...

  1. Close the Resolve3D window, then the main softWoRx window. Click the "x" in the top right corner of the windows.
  1. Upload your data to your file server, using the smb://filserver/yourname trick in the file browser window to get top your fileserver space.
  2. Log off the linux account via bottom left "Start" like icon > "Log off...".

[edit] If you are the last user...

  1. Turn off the Xenon lamp by clicking the little light bulb icon in the resolve 3D window.
  2. Then, first close the Resolve 3D window, then Main software window. In order to do this, click the "x" in the top right corner.
  1. Upload your data to your file server as described above
  2. Log out of the Linux computer and then shut it down.
  3. Turn off the instrument controller computer by pressing the round green button once.
    1. after 10 sec or so it will turn itself off auto magically.
    2. no need to touch the instrument controller software icon and manually shut down windows anymore!
  4. Turn off the green power switch (kills power to computers, camera and other stuff)
  5. Leave the weather station temperature controller ON at 25 (or 37 oC if the next user needs that)
Personal tools