MZ1 - Zeiss 2photon upright

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|link0=https://ifn.mpi-cbg.de/wiki/ifn/index.php/MPI-MZ1_-_Zeiss_2photon_upright_-_startup_%26_shutdown
 
|link1=https://spreadsheets.google.com/spreadsheet/ccc?key=0AiAvGnTuH4LedC1CZjIxa05nTnFOM2Ixb0RvdGltaEE&hl=en_US#gid=0
 
|link1=https://spreadsheets.google.com/spreadsheet/ccc?key=0AiAvGnTuH4LedC1CZjIxa05nTnFOM2Ixb0RvdGltaEE&hl=en_US#gid=0
|inv=AIM System 2501000445  10.1.35.33 (IPv4)
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|inv=AIM System 2501000445  10.1.145.41 (IPv4)
 
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Revision as of 15:57, 31 January 2012

  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
  • Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
  • Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
  • Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS.
MZ1-Zeiss 2photon upr.png


Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, 038C)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Imager, Upright stand, motorized XY stage, motorized Z-drive,
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss C-Apochromat 40x 1.2 W
Zeiss W Plan-Apochromat 40x/1.0
Zeiss W N-Achroplan 20x/0.5
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss LCI Plan-Neofluar 63x 1.3 Imm Corr
illumination
Fluorescence (Metal Halide, PRIOR, 200W)
Transmitted Light (Halogen)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Laser Multiphoton Chameleon 700-1064 nm
detection Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting

2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP and RFP

reflectors -
features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software Zen 2010 B
incubation Not available
links
  • [ beam path]
  • [ advanced system check]
inv.nr. AIM System 2501000445 10.1.145.41 (IPv4)



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