PhD Course Nov 09

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Contents

PhD Course Nov 09

Friday 13th Nov

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (Monday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:30 INTRODUCTION GROUP PRESENTATION
Wishes for the course
The ideal microsocpe
LMF TEAM
DEMO Optical Bench LMF TEAM
10:30 11:00 BREAK BREAK BREAK
11:00 12:30 LECTURE 1 Basics in Optics
Characteristics of light
Light sources
Reflection, Absorption, Refraction
Diffraction-Demo
Humberto
Practical 1
(Group Rotation)
1) Capillary + oil and Coin in glass
2) Laser refraction + reflection
3) Different immersion objectives - Demo Tirf Obective + DV oil box
1) Pete
2) Silke
3) Britta
12:30 13:30 LUNCH LUNCH LUNCH
13:30 15:45 Lecture 2 Introduction to lenses
Lens abberration
Understanding Ray optics
Humberto
Practical 2 Ray optics
Drawing ray diagrams
Practical 3
(Group Rotation)
1) Finding focal point of lenses + infinity + Colour fringes
2) Virtual and real images. 25cm distance
3) Image of Arrow in the microscope (upright and inverted)
1) Jan
2) Humberto
3) Pete + Britta
15:45 16:00 BREAK BREAK BREAK
16:00 17:00 Lecture 3 Microscope components
Focusing, Field of View, Depth of field, depth of focus
Planes (filament Demo)
Conjugated planes
Koehler illumination
Humberto
17:00 18:30 Practical 4 Glass cube demo and practise
Focusing; Depth of field / focus
Koehler illumination
LMF TEAM


DAY TWO (TUESDAY)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 INTRODUCTION
(groups of 3)
Koehler Exercise
Questions
LMF TEAM
10:00 11:25 Practical 5 Diffraction grids / gratings
LECTURE 4a Diffraction
The Eye
Interference
Humberto
Practical 6 Airy disk on the microscope through mirror LMF TEAM
LECTURE 4b Resolution
NA
Humberto
11:25 11:40 BREAK BREAK BREAK
11:40 12:40 Practical 7 1) NA Objectives - angle measurements
2) Diffraction Exercise on microscope
3) Fluorescent beads with variable NA
1) Silke
2) Dan
3) Britta
12:40 13:40 LUNCH LUNCH LUNCH
13:40 15:30 Lecture 5a Contrasting techniques I
Dark field
Humberto
Practical 8a
(4 groups)
Dark field LMF TEAM
Lecture 5b Contrasting techniques I
Phase contrast
Humberto
Practical 8b
(4 groups)
Phase contrast LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 17:45 Lecture 6a Contrasting techniques II
Polarization
Humberto
Practical 9a
(4 groups)
Polarization LMF TEAM
Lecture 6b Contrasting techniques II
Differential Interference Contrast
Humberto
Practical 9b
(4 groups)
DIC LMF TEAM


DAY THREE (Wednesday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:45 INTRODUCTION
(groups of 3)
Questions LMF TEAM
9:45 10:30 LECTURE 7 Objectives
Objective correction
Infinity optics
Humberto
10:30 10:45 BREAK BREAK BREAK
10:45 11:45 Practical 10 1) Lens Abberration
2) Objective reading and cleaning
3) Infinity optics on optical bench, coverslip thickness measurement
1) Humberto
2) Silke
3) Jan
11:45 12:30 LECTURE 8 Basics of Fluorescence
Fluorochromes
Dan
12:30 13:30 LUNCH LUNCH LUNCH
13:30 15:00 LECTURE 9 (interactive) Fluorescence Microscopy
Filters
Silke
Practical 11 Mercury lamp exchange and alignment
Fluorescence imaging
LMF TEAM
15:00 15:45 Lecture 10 CCD Britta
15:45 16:00 BREAK BREAK BREAK
16:00 16:45 Lecture 11 Digital Imaging Dan
16:45 18:30 Practical 12 (4 groups) Wide field systems + CCD LMF TEAM


Checklist for Widefield/CCD practical

  • Exposure Time
    • Gain
    • Binning
    • ROI
    • Saturation
    • Histogram
    • Gamma
  • Mapping/LUTs
  • Images - Display
  • Scale - ruler
  • Pixel size


DAY FOUR (Thursday)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 INTRODUCTION
(groups of 3)
Questions
Filter reading
LMF TEAM
9:30 09:45 DEMO - CCD - Colour Britta
9:45 10:15 LECTURE 12a Optical sectioning Dan
10:15 10:30 BREAK BREAK BREAK
10:30 11:45 LECTURE 12b Confocal Microscopy
Multiphoton microscopy
Spinning disc
TIRF
Dan
11:45 12:45 LUNCH LUNCH LUNCH
12:45 14:15 Practical 13a SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision
Pete, Britta, Dan
14:15 14:30 BREAK BREAK BREAK
14:30 16:00 Practical 13b SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision
Pete, Britta, Dan
16:00 17:00 / 16:15 BREAK SEMINAR / BREAK BREAK
17:00 / 16:15 18:30 / 17:45 Practical 13c SYSTEM ROTATION
Leica confocal, Spinning disc, Delta Vision
Pete, Britta, Dan


DAY Five (Friday)

Please bring your own laptop and preinstall Fiji!!!

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 10:00 INTRODUCTION
(groups of 3)
Questions LMF TEAM
10:00 11:30 LECTURE 13a Digital Images
What is image processing
Dan
Practical 14 Fiji (... is just ImageJ)
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 LECTURE 13b Image processing Dan
Practical 14 Fiji (... is just ImageJ)
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 LMF booking database
IFN concept, members and Wiki
Final discussion
IFN
15:30 16:00 Evaluation LMF TEAM


Office Duty

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
Morning first break / Britta Dan Pete Britta
first break lunch Dan Pete Britta Humberto Silke
lunch break Silke Silke Pete Humberto
Silke
/
break end Pete Dan Humberto Humberto
Silke
/

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