SP5 I, upright, BIOTEC

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|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
 
|Institute-logo=Biotec logo.png
 
|Institute-logo=Biotec logo.png
|description=Our [[Leica TCS SP5]] I [[confocal]] system is designed for routine work and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
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|description=Our [[Leica TCS SP5]] I [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|applications=-
 
|applications=-
 
|image=Bioz02.JPG
 
|image=Bioz02.JPG
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|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation
 
|features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation
 
|software=LAS AF
 
|software=LAS AF
|incubation=Not available
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|incubation=Cage incubator (T+CO2)
 
|link0=http://www.biotec.tu-dresden.de/fileadmin/technology/imaging/Leica_SP5_upright_I_-_manual.pdf
 
|link0=http://www.biotec.tu-dresden.de/fileadmin/technology/imaging/Leica_SP5_upright_I_-_manual.pdf
 
|link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_SP5I
 
|link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_SP5I

Latest revision as of 12:46, 22 May 2015

Suitable for -.
Bioz02.JPG


[edit] Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 220)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Leica - DM6000, upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC
objectives
Leica HCX PL APO 40x 0.75
Leica HC PL APO 20x 0.7
Leica HC PL APO 10x 0.4
Leica HC PL FLUOTAR 5x 0.15
Leica HC PL APO CS 40x 1,25-0,75 Oil
Leica HC PL APO 100x 1.4 Oil
Leica HCX PL APO 100x 1.47 Oil Korr.
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, HXP, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 496, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
detection point scanner, 2 confocal HyD´s, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole
reflectors GFP/FITC (L5) : EX 480/40 ; BS 505 ; EM BP 527/30

DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425
TRITC/DsRed/RFP : EX 545/30 ; LP 570 ; EM BP 620/60
Analysator

features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation
software LAS AF
incubation Cage incubator (T+CO2)
links
  • [- beam path]
  • [ advanced system check]
inv.nr. 146330



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