SP5 II, upright, CRTD

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|category=Laser Scanning Confocal
 
|category=Laser Scanning Confocal
 
|facility=Imaging@BIOTEC/CRTD
 
|facility=Imaging@BIOTEC/CRTD
|building=CRTD
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|building=BIOTEC
|room=-1.121
+
|room=220
 
|Facility-logo=Imaging@Biotec.jpg
 
|Facility-logo=Imaging@Biotec.jpg
|Institute-logo=CRTD logo.PNG
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|Institute-logo=Biotec logo.png
 
|description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.
 
|applications=-
 
|applications=-

Latest revision as of 10:05, 2 November 2017

Suitable for -.
SP5 II, upright, CRTD.jpg


[edit] Directions

Biotec logo.png
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (BIOTEC, 220)

[edit] Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

[edit] Details

microscope Leica - DM6000, Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC
objectives
Leica HC PL APO CS 10x 0.4
Leica HC PL APO CS 20x 0.7
Leica HCX PL APO 40x 0.75
Leica HCX PL APO 63x 1.2 W
Leica HC PL APO 100x 1.4 Oil
Leica HCX APO L 20x 0.5 W
Leica HCX APO L 40x 0.8 W
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, HXP, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 496, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
detection point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole
reflectors DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425

GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40
TRITC (N2.1) : EX 537/45 ; BS 580 ; EM LP 590
Analysator

features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP
software LAS AF
incubation Cage incubator (T+CO2)
links
  • [- beam path]
  • [ advanced system check]
inv.nr. 71927



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