Sample Preparation for Electron Microscopy - Full List
From BioDIP
| Technique | Name | Description | Facility | |
|---|---|---|---|---|
| Nanofilm TEM Stainer, CRTD | Automated contrasting device | Nanofilm TEM Stainer, CRTD | automated contrasting of ultrathin sections on grids | EM- and Histo-Facility CRTD / BIOTEC |
| Leica AFS, MPI | Automated freeze-substitution | Leica AFS, MPI | Automated freeze-substitution and low temperature embedding. | MPI-CBG EM |
| Leica AFS2, MPI | Automated freeze-substitution | Leica AFS2, MPI | Automated freeze-substitution and low temperature embedding. | MPI-CBG EM |
| Leica AFS2, CRTD | Automated freeze-substitution | Leica AFS2, CRTD | for freeze substitution and low temperature embedding embedding | EM- and Histo-Facility CRTD / BIOTEC |
| Baltec MED-020, MPI | Carbon evaporator | Baltec MED-020, MPI | carbon coating and glow discharge of EM grids (also used for glow discharge) | MPI-CBG EM |
| Baltec MED-020, CRTD | Carbon evaporator | Baltec MED-020, CRTD | carbon coating and glow discharge of EM grids, with additional sputter coating device | EM- and Histo-Facility CRTD / BIOTEC |
| Baltec CPD 030, BIOTEC | Critical point dryer | Baltec CPD 030, BIOTEC | drying of samples for SEM-inspection | EM- and Histo-Facility CRTD / BIOTEC |
| Leica CPD 300, CRTD | Critical point dryer, automated | Leica CPD 300, CRTD | drying of samples for SEM-inspection, fully automated | EM- and Histo-Facility CRTD / BIOTEC |
| Keyence BZ 8000, CRTD | Epifluorescence microscope | Keyence BZ 8000, CRTD | widefield fluorescence microscope for CLEM (equipped with color camera) | EM- and Histo-Facility CRTD / BIOTEC |
| Leica MZ6F, CRTD | Epifluorescence microscope | Leica MZ6F, CRTD | target preparation | EM- and Histo-Facility CRTD / BIOTEC |
| Leica EMGP, MPI | Grid plunger | Leica EMGP, MPI | Rapid freezing of thin layers (for single particle analysis in cryoEM). | MPI-CBG EM |
| Baltec HPM-010, MPI | High-pressure freezer | Baltec HPM-010, MPI | High-pressure freezing of samples up to 2mm in diameter (for bigger samples). | MPI-CBG EM |
| Leica EMPACT2+RTS, MPI | High-pressure freezer | Leica EMPACT2+RTS, MPI | High-pressure freezing of samples with rapid transfer (For time-resolved CLEM). | MPI-CBG EM |
| Leica IGL, CRTD | Immunogold labeling automat | Leica IGL, CRTD | automated immunogold labeling of sections (for serial section immuno-EM) | EM- and Histo-Facility CRTD / BIOTEC |
| Leica EM-KMR, CRTD | Knifemaker | Leica EM-KMR, CRTD | production of glass knifes | EM- and Histo-Facility CRTD / BIOTEC |
| PELCO Biowave Pro, MPI | Microwave processor | PELCO Biowave Pro, MPI | accelerated sample preparation from fixation to infiltration | MPI-CBG EM |
| Leica EM-AMW, CRTD | Microwave processor | Leica EM-AMW, CRTD | microwave assisted automated sample preparation (for epon or Technovit embedding) | EM- and Histo-Facility CRTD / BIOTEC |
| Baltec SCD 050, BIOTEC | Sputter Coater | Baltec SCD 050, BIOTEC | coating of dry samples with conductive material | EM- and Histo-Facility CRTD / BIOTEC |
| Leica EM-TP, CRTD | Tissue processor | Leica EM-TP, CRTD | Automated sample preparation (for epon or Technovit embedding) | EM- and Histo-Facility CRTD / BIOTEC |
| Leica UC6-FCS, CRTD | Ultacryomicrotome | Leica UC6-FCS, CRTD | Tokuyasu cryosections | EM- and Histo-Facility CRTD / BIOTEC |
| Leica UC6-FC6, MPI | Ultacryomicrotome | Leica UC6-FC6, MPI | for Tokoyasu sections | MPI-CBG EM |
| Leica UCT, MPI-1 | Ultramicrotome | Leica UCT, MPI-1 | for plastic embedded samples | MPI-CBG EM |
| Leica UCT, MPI-2 | Ultramicrotome | Leica UCT, MPI-2 | for plastic embedded samples | MPI-CBG EM |
| Leica UCT, MPI-3 | Ultramicrotome | Leica UCT, MPI-3 | for plastic embedded samples | MPI-CBG EM |
| Leica UC6, CRTD | Ultramicrotome | Leica UC6, CRTD | sectioning of plastic embedded samples | EM- and Histo-Facility CRTD / BIOTEC |
