W1 - Olympus TIRF

Suitable for membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics.

Directions

MPI-CBG LMF (MPI-CBG, U32)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX71, Inverted stand, manual, manual XY stage
objectives	Olympus UPlanSApochromat 10x 0.4 Olympus MPlanFluarN 100x 0.9 Olympus PlanApochromat 100x 1.45 Oil TIRFM Olympus UApochromat 150x 1.45 Oil TIRFM Olympus Apo N 100x 1.70 HIOil TIRFM
illumination	Transmitted Light (Halogen) Fluorescence (Metal Halide, X-Cite, 120W) Laser DPSS 491 nm Laser DPSS 561 nm
detection	[[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet; pixel size of EMCCD chip: 16um; image pixel size (measured June 29th, 2012): with 10x objective: 1.592um/px (1x optovar); 0.795um/px (2x optovar); with 100x objective: 0.159um/px (1x optovar); 0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar); 0.052um/px (2x optovar)]]
reflectors	Hamamatsu W-View image splitter: two matching dichromatic mirrors: 590LP + 590SP; five emitters: 520/15, 525/30, 617/73, 628/40, Z491/561 M-TIRF microscope filter turret: position 1: for TIRF 488Ex/DC500/LP505Em, 2: for TIRF Z488Ex/DCZDC488/BP 520/40, 3: empty, 4: tripple DC for 491, 561 and 640 laser excitation, no emitter!, 5: dual Ex and DC for 491 and 561 laser excitation, no emitter!, 6: only DC and EM LP 575
features	custom-built TIRF condenser, manual TIRF angle adjustment
software	[[software::iQ 2.9 + iQ 3.2 iQ3 user guide with Python IDE]]
incubation	Stage incubator (T+CO2) + objective heater
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.	404504 10.1.145.13 AIM 2601000564"404504 10.1.145.13 <br /> AIM 2601000564" cannot be used as a page name in this wiki.

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