W2 - DeltaVision Core

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Suitable for Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy..
W2 - DeltaVision Core.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, U35)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - IX71, Inverted stand, manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control.
objectives
Olympus UPlan SApochromat 10x 0.4
Olympus UPlan SApochromat 20x 0.75
Olympus UApochromat/340 40x 1.35 Oil
Olympus Plan ApochromatN 60x 1.42 Oil
Olympus UPlan SApochromat 100x 1.4 Oil
Olympus UApochromat 150x 1.45 Oil TIRFM
Olympus UPlan Apochromat 60x 1.20 Water
Olympus UPlan SApochromat 100x 1.4 Oil
illumination
SSI-lumencor
Transmitted Light (LED)
detection Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php
reflectors [[reflectors::Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5

see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]]

features Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki.
software Applied Precision SoftWorx running on a Cent OS Linux pc. Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system.
incubation Cage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZGO 4076530000 10.1.145.25 cx63541s



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