W2 - DeltaVision Core

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Suitable for Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy..
MPI-W2.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - IX71, Inverted stand, manual, with Applied Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control.
objectives
Olympus UPlanSApo 10x 0.4
Olympus UPlanSApo 20x 0.75 0.65mm WD
Olympus UApo/340 40x 1.35 Oil Iris
Olympus PlanApo N 60x 1.42 Oil UIS2 inf/0.17/FN26.5 WD 0.15 mm
Olympus UPlanSApo 100x 1.4 Oil
Olympus UApo 150x 1.45 Oil TIRFM inf/0.13-0.21 corr
Olympus UPlanApo 60x 1.20 Water PSF WD 0.25 mm
illumination
SSI-lumencor
Transmitted Light (LED)
detection Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php
reflectors [[reflectors::Separate excitation selection, dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera. Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5, 2) GFP/mCherry, 3) CFP/YFP, 4) DAPI/FITC/A594/Cy5

see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]]

features Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki.
software Applied Precision SoftWorx running on a Cent OS Linux pc. Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system.
incubation Cage incubator (T+CO2)
links
  • [ beam path]
  • [ advanced system check]
inv.nr. MOZGO 4076530000 10.1.145.25 cx63541



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