ApoTome2, inverse, CRTD

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Suitable for multichannel fluorescence, 3-D, time-lapse, tile scan, multiple points, hardware autofocus.
ApoTome2, inverse, CRTD.jpg


Directions

CRTD logo.PNG
Imaging@Biotec.jpgImaging@BIOTEC/CRTD (CRTD, -1.124)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope Zeiss - Axio Observer.Z1, Inverted stand, automatic XY stage, motorized z-stage, fluorescence, transmitted light with manual DIC (Senarmont), b/w epifluorescence acquisition, Optical Sectioning (ApoTome2), Mosaic (tile scans)
objectives
Zeiss EC Plan-Neofluar 10x 0.3 Ph1
Zeiss LD Achroplan 20x 0.4 Ph2
Zeiss EC Plan-Neofluar 40x 0.6 Ph2
Zeiss C-Apochromat 63x 1.2 W
Zeiss EC Plan Neofluar 2.5x 0.075
Zeiss Plan-Apochromat 20x 0.8
illumination
Fluorescence (LED, 470nm)
Fluorescence (LED, 590nm)
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
detection Axiocam HRc (1388*1040, 6,45*6,45µm pixel) - color

Axiocam MRm (1388*1040, 6,45*6,45µm pixel) - b/w
tube lens 1x and 1.6x

reflectors FS 49: EX BP 347/55; BS 395; EM BP 445/50 (Dapi)

FS 38HE: EX BP 471/40 (HE); BS 495 (HE); EM BP 495-545 (HE); (GFP)
FS 43HE: EX BP 550/25 (HE); BS 570 (HE); EM 605/70 (HE); (Cy3)
FS Cy5: EX BP 662-738; BS 660; EM BP 700/75; (Cy5)
FS F56-422: EX BP 474/23 & BP 585/29; BS 495-555 & BP 610-690; EM BP 525/55 & BP 645/60; (GFP-DsRed)
FS Analysator for DIC and Transmission

features -
software 32 bit: AxioVision

64 bit: Win7 ZEN blue 2012"32 bit: AxioVision 64 bit: Win7 ZEN blue 2012" cannot be used as a page name in this wiki.

incubation Not available
links
  • [ manual]
  • [ laser power values]
  • [ beam path]
  • [ advanced system check]
inv.nr. 70836



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