BasicsCoursePhDprog-PeterEvennetMay2011
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Contents |
PhD Program: Basics of Light Microscopy - Peter Evennett / LMF - Course May 2011
Setup -Fri 13 May
Setup - all lmf staff move stuff to galleria Equipment needed
- Microscopes
- Optical bench
- Spinning disk gadget
- Polarazing sheets
- Diffraction Grids
- Fluorescent samples
- Fluorescent Beads (Prepare slides)
- First day Handout for students
- Light torches
DAY ONE (16th May)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 09:30 | FINAL PREP | Test everything works | LMF TEAM |
| 9:30 | 10:30 | INTRODUCTION | COURSE INTRO WHO is WHO? Round table |
LMF TEAM |
| 10:30 | 11:00 | Session 1 | Historical aspects of light microscopy | Peter Evennett |
| 11:00 | 11:15 | BREAK | BREAK | BREAK |
| 11:15 | 12:30 | SESSION 1 Theory |
PRINCIPLES OF LIGHT MICROSCOPY Historical aspects of microscopy Introduction to lenses (Lens demo fun) Lens aberrations (see colour fringes of lighting image with simple lens) Magnification Numerical aperture Microscope illumination Conjugate planes |
PETER EVENNETT LMF TEAM |
| 12:30 | 13:30 | LUNCH | LUNCH | LUNCH |
| 13:30 | 14:15 | SESSION 1 Practice Rotation |
Milky Glass Block demo Conjugate planes on bench microscope NA measurements |
Dan Jan Silke |
| 14:15 | 15:30 | SESSION 2 Theory |
Koehler Illumination (Microscope alignment) |
PETER EVENNETT |
| 15:30 | 15:45 | BREAK | BREAK | BREAK |
| 15:45 | 18:00 | SESSION 2 Practice |
Koehler illumination (Microscope alignment) |
PETER EVENNETT LMF TEAM |
DAY TWO (May 17th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:45 | Recap | day 1 | LMF TEAM |
| 9:45 | 11:00 | SESSION 1 Theory & Practice |
Resolution Diffraction |
PETER EVENNETT LMF TEAM |
| 11:00 | 11:15 | BREAK | COFFEE BREAK | |
| 11:15 | 13:15 | DEMONSTRATION | Abbe´s diffraction experiments |
PETER EVENNETT LMF TEAM |
| 13:15 | 14:15 | BREAK | LUNCH | |
| 14:15 | 15:15 | SESSION 2 Theory |
Bright Field Dark Field |
PETER EVENNETT LMF TEAM |
| 15:15 | 16:15 | SESSION 2 Practice |
Bright Field Dark Field |
PETER EVENNETT LMF TEAM |
| 16:15 | 16:30 | BREAK | COFFEE BREAK | |
| 16:30 | 17:15 | SESSION 2 Theory |
Phase Contrast microscopy | PETER EVENNETT LMF TEAM |
| 17:15 | 18:15 | SESSION 2 Practice |
Phase Contrast microscopy | PETER EVENNETT LMF TEAM |
DAY THREE (May 18th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:45 | Recap | day 2 | LMF TEAM |
| 9:45 | 10:45 | SESSION 1 Theory |
Objective reading Objective cleaning |
PETER EVENNETT LMF TEAM |
| 10:45 | 11:00 | BREAK | BREAK | BREAK |
| 11:00 | 12:00 | SESSION 1 Practice Group rotation |
Cover glasses and sample mounting Objective reading Objective cleaning |
Dan Britta, Silke Jan |
| 12:00 | 13:15 | SESSION 2 Theory & Practice |
Polarized light microscopy | PETER EVENNETT LMF TEAM |
| 13:15 | 14:15 | BREAK | LUNCH | |
| 14:15 | 15:30 | SESSION 3 Theory & Practice |
Differential Interference Contrast (DIC) | PETER EVENNETT LMF TEAM |
| 15:30 | 15:45 | BREAK | BREAK | BREAK |
| 15:45 | 16:15 | Question round | Questions to Peter Evennett | PETER EVENNETT LMF TEAM |
| 16:15 | 18:15 | SESSION 4 Theory & Demo |
What is a pixel Analog to Digital conversion Nuyquist-Shannon |
Dan |
DAY FOUR (May 19th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) | DEMO Activities | DEMO materials |
| 9:00 | 9:45 | Recap | day 3 | LMF TEAM | ||
| 9:45 | 10:30 | SESSION 1 Theory |
Fundamental concepts of fluorescence | LMF TEAM Dan |
- | - |
| 10:30 | 10:45 | BREAK | BREAK | BREAK | BREAK | BREAK |
| 10:45 | 12:15 | SESSION 2 Theory & Practice |
Fluorescence Microscopy | LMF TEAM Silke |
• Light sources • Lamp houses • Spectra • Filters • Filter cubes • Filter spectral charts of each microscope (laminated) |
• Spare lamp houses • Lamps • Ocean optics equipment • Four (4) different Filter-sets (cubes), with the appropriate descriptions • diagramms for drawing filter/fluorophore spectra |
| 12:15 | 13:15 | SESSION 2 Practice |
Fluorescence imaging at microscopes | LMF TEAM | - | • Fluorescent labeled samples |
| 13:15 | 14:15 | BREAK | LUNCH | LUNCH | LUNCH | LUNCH |
| 14:15 | 15:45 | SESSION 3 Theory & Practice |
Imaging with CCD and Quantitative Imaging | LMF TEAM Britta/Dan |
• CCD • Cameras |
• CCD chip • RGB Slider Spot Camera setup |
| 15:45 | 16:00 | BREAK | BREAK | |||
| 16:00 | 18:00 | SESSION 4 Practice |
Imaging with CCD cameras using LMF systems | LMF TEAM | • Displaying • Pixel size • Look up table • Binning • Exposure time • Saturation • Signal to noise • ROI • Bit depth • Auto map • Auto contrast |
• Microscopes: DVcore(Dan, 3 students) pDV (Silke, 2 students) Inverted CCD (Pete, 2 students) N-Storm (Britta, 2 students) self-built-syst (Jan, 3 students)) • Fluorescence Sample slides (Kidney / Cells) |
| 19:00 | open end | Dinner | with Peter | LMF TEAM |
DAY FIVE (May 20th)
| FROM | TO | ACTIVITY | TOPICS | Responsible person(s) |
| 9:00 | 9:45 | Recap | day 4 | LMF TEAM |
| 9:45 | 10:45 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
| 10:45 | 11:00 | BREAK | BREAK | BREAK |
| 11:00 | 13:15 | SESSION 1 Theory & Demo |
Optical sectioning methods Pros&Cons |
LMF TEAM Dan / Jan |
| 13:15 | 14:15 | BREAK | LUNCH | |
| 14:15 | 15:00 | SESSION 2 Small Groups Discussion |
What techniques might be good for me (Own Projects) |
LMF TEAM Dan/Jan/Silke/Britta/Pete? |
| 15:00 | 15:15 | BREAK | BREAK | BREAK |
| 15:15 | 16:00 | SESSION 3 | Course evaluation | LMF TEAM |
OFFICE DUTIES
- LMF Members available for users (16th May to 20th May) -
No office duty this time due to lack of staff. One member of the LMF will always carry the helpline phone, though.
| FROM | TO | MONDAY | TUESDAY | WEDNESDAY | THURSDAY | FRIDAY |
| 9:30 | 12:30 | - | --- | --- | --- | --- |
| 12:30 | 13:30 | L | U | N | C | H |
| 13:30 | 18:00 | --- | --- | --- | --- | - |
