Live Cell/TIRF AF6000, BIOTEC

Suitable for -.

Directions

Imaging@BIOTEC/CRTD (BIOTEC, 226)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope	Leica - DMI6000, Inverted stand, motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF
objectives	Leica HC PL APO 10x 0.4 Leica HC PL APO 20x 0.7 Leica HCX PL APO 40x 0.85 Leica HC PL APO 100x 1.46 Oil
illumination	Laser Diode 405 nm Laser DPSS 488 nm Laser DPSS 561 nm Laser HeNe 633 nm Fluorescence (Metal Halide, HXP, 120W) Transmitted Light (Halogen)
detection	Andor iXON 897 EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD
reflectors	FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30 CY5 : EX 635/10 ; BS 650 ; EM BP 720/60 Quad Laser Line VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65 RFP : EX 552/24 ; BS 570 ; EX 605/65
features	sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD)
software	LAS AF
incubation	Cage incubator (T+CO2)
links	[ manual] laser power values [- beam path] [ advanced system check]
inv.nr.	71925

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