CO1 - Olympus Fluoview 1000

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Suitable for ablation experiments.
MPI09.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038A)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Olympus - BX 61, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC
objectives
Olympus UPlanSApochromat 10x 0.4
Olympus UPlanSApochromat 40x 0.9
Olympus UPlanSApochromat 20x 0.85 Oil
Olympus UApochromat 40x 1.35 Oil
Olympus UPlanSApochromat 60x 1.35 Oil
Olympus UPlanApochromat 60x 1.2 W
illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser Diode 405 nm
Laser Argon Multiline 458, 477, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Laser Diode 405 nm (pulsed)
detection [[detection::point scanner, 4 confocal PMTs, two independent spectral detection channels, each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection, two filter based detection channels, T-PMT, dichromatic mirrors for excitation/emission splitting, one pinhole]]
reflectors [[reflectors::Filter Cube 1. U-MWU2. BP330-385 BA420 DM400

Filter Cube 2. U-MWB2. BP460-490 BA520IF (High performance interference filter) DM500
Filter Cube 3. U-MWG2. BP510-550 BA590 DM570]]

features Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!

Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching"Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database! Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation, laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant), sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, Kalman averaging, summarizing, scan zoom and rotation; advanced use of motorized stage due to MATL-software, which allows independent multi-point acquisition and tiling + some simple stitching" cannot be used as a page name in this wiki.

software FV10-ASW 1.7
incubation Stage incubator (T+CO2)
links
inv.nr. MOZG0407421000 10.1.38.205



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