D1 - SPIM Demo

Suitable for developmental studies on living embryos, spheroids, cleared tissues, etc..

Directions

[[image:|150px|text-bottom|right]]

[[image:|100px]] MPI-CBG LMF (MPI-CBG, -341)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Zeiss prototype SPIM 3D stand, 3D stand, imaging chamber, light sheet illumination. Various Zeiss Water dipping and air lenses (no 40x)
objectives	Zeiss W N-Achroplan 20x/0.5 Zeiss N-Achroplan 63x 0.9 W
illumination	Transmitted Light (LED) Fluorescence (HBO) Laser Argon Multiline 458, 477, 488, 514 nm Laser DPSS 561 nm Laser HeNe 633 nm
detection	Zeiss CCD Axiocam camera. Max 3fps full chip. Faster with binning.
reflectors	50/50 for sample location/lightsheet alignment etc., BrightLine HC FITC F36-501, Green Fset09, BrightLine HC mCherry 624/40 F37-624 emitter only, YFP FSet46he, CFP FSet51, BrightLine HC Green 514/30,
features	Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry."Its got a lightsheet and perpendicular detection with water dipping lenses, from a sample embedded in agarose suspended in watery medium. It has a non standard stand so field of view with a particular objective is not same as a conventional microscope. No 405/UV spim illumination sorry." cannot be used as a page name in this wiki.
software	Zeiss AIM (SPIM modifications/macro), Fiji, BioImageXD. Remote control possible.
incubation	Stage incubator (T)
links	[ manual] [- laser power values] [- beam path] [ advanced system check]
inv.nr.	0 10.1.235.28

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