H2 - Nikon STORM - TIRF

Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging.

Directions

MPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Nikon - Eclipse Ti, Inverted stand, motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens; motorized TIRF-condenser with additional manual collimating lens"motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;<br />motorized TIRF-condenser with additional manual collimating lens" cannot be used as a page name in this wiki.
objectives	Nikon Plan-Apochromat 10x 0.45 DIC Nikon Plan-ApochromatVC 60x 1.2 Water DIC Nikon Apochromat 60x 1.49 Oil DIC Nikon ApochromatVC 100x 1.4 Oil DIC Nikon SR-Apochromat 100x 1.49 Oil DIC Nikon Planfluor 20x/0.75 MImm
illumination	Transmitted Light (Halogen) Fluorescence (LED) Laser Diode 405 nm Laser DPSS 488 nm Laser DPSS 561 nm Laser DPSS 640 nm
detection	at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD SN #4431 with cylindrical lens; dexel size of camera chip: 16um at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD # 6499 with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um
reflectors	Microscope stand: reflector revolver FL2 (lower floor): position 1: L405 cube (EX: BL 406/15, DC: 425DCXR, EM: BL HC 457/50), 2: L470 cube (EX: ET 470/40, DC: 495 LP, EM: ET 525/30), 3: Dual LGFP/mCherry cube (EX: dual-band 475/30 + 570/30, DC: dual-band TM GFP/mCherry, EM dual-band 520/40 + 630/60), 4: narrowGFP (only EM 510/40), 5: L620 cube (EX: ET BP 620/60, DC: 660 LPXR, EM: BP 700/75), 6: empty reflector revolver FL1 (upper floor): position 1: TIRF1 cube (quad DC: zt405/488/561/640rpc + quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 2: TIRF2Q cube (quad DC: zt 405/488/561/640, quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28), 3: HC YFP cube (EX: BL HC 500/24, DC: HC BS 520LP, EM: BL HC 542/27), 4: L565 cube (EX: BL HC 562/40, DC: BS 593, EM: BL HC 624/40), 5: Analyzer, 6: empty Sutter filter wheel: position 0: BrightLine 617/73, 1: Razor Edge LP488RS, 2: Razor Edge LP664RS, 3: BrightLine 447/60, 4: open, 5: Dual BrightLine 512/630 (512/23 + 630/91), 6: BrightLine 628/40, 7: BrightLine 525/30, 8: BrightLine 607/36, 9: BrightLine 520/15 Hamamatsu W-View image splitter: for bypass mode: silver mirror for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100, reflection 615/90) and 560 DCLP (reflection 500/100, transmission 635/130), emission filters: ET BP 535/30, BL HC 628/40
features	-
software	NIS Elements 4.51.01 with N-STORM module + Jobs module
incubation	Cage incubator (T+CO2)
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.	0 10.1.32.156

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