MZ1 - Zeiss 2photon upright

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Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS.
MZ1 - Zeiss 2photon upright.jpg


Directions

Logo MPI-CBG.jpg
MPI-CBG-Lmf-logo-big.pngMPI-CBG LMF (MPI-CBG, 038C)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope Zeiss - Axio Examiner.Z1, Upright stand, motorized XY stage, motorized Z-drive,
objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss Plan-Apochromat 10x 0.45
Zeiss Clr Plan-Apochromat 10x 0.5 Scale
Zeiss N-Achroplan 20x 0.5 W
Zeiss Plan-Apochromat 20x 0.8
Zeiss W Plan-Apochromat 20x 1.0 DIC
Zeiss Clr Plan-Neofluar 20x 1.0 Korr Clarity
Zeiss LD SC Plan-Apochromat 20x 1.0 Korr Scale
Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
Zeiss C-Apochromat 40x 1.2 W
illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Laser Diode 405 nm
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Laser Multiphoton Chameleon 700-1064 nm
detection Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting

2 photon detection: 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD moule) in transmitted direction

reflectors Zeiss set 49: DAPI

Zeiss set 38 Endow: GFP
Zeiss set 45: HQ Texas Red

features simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan
software Zen 2012 black
incubation Not available
links
  • [ beam path]
  • [ advanced system check]
inv.nr. AIM System 2501000445 10.1.145.41 (IPv4)



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