Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan..

Directions

MPI-CBG LMF (MPI-CBG, 456A)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope

Zeiss - Axio Observer, Inverted stand, motorized XY stage, motorized Z-drive,

objectives

Zeiss Plan-Apochromat 10x 0.45

Zeiss C-Apochromat 40x 1.2 W

Zeiss Plan-Apochromat 20x 0.8

Zeiss Plan-Apochromat 63x 1.4 Oil

Zeiss LD C-Apochromat 40x 1.1 W

Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC

Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC

illumination

Fluorescence (Metal Halide, PRIOR, 200W)

Transmitted Light (Halogen)

Laser Diode 405 nm

Laser Argon Multiline 458, 477, 488, 514 nm

Laser DPSS 561 nm

Laser HeNe 594 nm

Laser HeNe 633 nm

Laser Multiphoton Chameleon 700-1064 nm

detection

[[detection::Single photon detection: 2 confocal PMTs, QUASAR detector with 32 channels, T-PMT, dichromatic mirrors for excitation/emission splitting, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting

2 photon detection: 2 non-descanned detectors (NDD) - NDD-dichromatic mirrors and emission filters]]

reflectors

-

features

simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, spectral scan

software

ZEN 2010

incubation

Not available

links