PeterEvennettCourse04-2010

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Contents

Peter Evennett's Course April 2010

Setup -Fri 16th Apr

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (19th Apr)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:05 INTRODUCTION COURSE PRESENTATION
GROUP PRESENTATION
LMF TEAM
11:05 11:20 BREAK BREAK BREAK
11:20 13:00 SESSION 1
Theory & Practice
PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects of microscopy
Introduction to lenses
Lens aberrations
Magnification
Numerical aperture
Microscope illumination
Conjugate planes
Koehler illumination
PETER EVENNETT
LMF TEAM
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 SESSION 2
Theory & Practice
PRINCIPLES OF LIGHT MICROSCOPY
Demo
PETER EVENNETT
LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 18:00 SESSION 3
Theory & Practice
PRINCIPLES OF LIGHT MICROSCOPY PETER EVENNETT
LMF TEAM

DAY TWO (Apr 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 1 LMF TEAM
9:30 10:45 SESSION 1
Theory & Practice
Resolution
Diffraction
PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK COFFEE BREAK
11:00 13:00 DEMONSTRATION Abbe´s diffraction experiments
PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 16:00 SESSION 2
Theory & Practice
Bright Field
Dark Field
Phase Contrast microscopy
PETER EVENNETT
LMF TEAM
16:00 16:15 BREAK COFFEE BREAK
16:15 18:00 SESSION 2
Theory & Practice
Bright Field
Dark Field
Phase Contrast microscopy
PETER EVENNETT
LMF TEAM

DAY THREE (Apr 21th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 2 LMF TEAM
9:30 11:30 SESSION 1
Theory & Practice
Objective reading
Objective cleaning
PETER EVENNETT
LMF TEAM
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 SESSION 2
Theory & Practice
Polarized light microscopy
Differential Interference Contrast (DIC)
PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 3
Theory & Practice
Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy
PETER EVENNETT
LMF TEAM
15:15 15:30 BREAK BREAK BREAK
15:30 18:00 SESSION 4
Theory & Practice
Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy
PETER EVENNETT
LMF TEAM

DAY FOUR (Apr 22th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:00 9:30 Recap day 3 LMF TEAM
9:30 10:20 SESSION 1
Theory
Question session
Fundamentals concepts of fluorescence
LMF TEAM
Dan
- -
10:20 10:55 BREAK BREAK BREAK BREAK BREAK
10:55 13:15 SESSION 2
Theory & Practice
Fluorescence Microscopy LMF TEAM
Dan? Sike? Jan?
• Mercury/Xenon/MetalHalide lamp
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Fluorescent-labeled samples
• Filter spectral charts of each microscope (laminated)
• Spare lamp houses
• Lamps
• Ocean optics equipment
• Different filters
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
13:15 14:15 BREAK LUNCH LUNCH LUNCH LUNCH
14:15 15:45 SESSION 3
Theory & Practice
Imaging with CCD and Quantitative Imaging LMF TEAM
Britta/Dan
• CCD
• Cameras
• CCD chip
• RGB Slider Spot Camera setup
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice
Imaging with CCD cameras using LMF systems LMF TEAM
Jan / Peter / Dan / Britta / Silke (*)
• Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast
• Microscopes (DVcore, Inverted CCD, Upright CCD, polychrome, Teaching FM
• Fluorescence Sample slides (Kidney / Cells

DAY FIVE (October 23th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:00 9:30 Recap day 4 LMF TEAM
9:30 10:20 SESSION 1
Theory & Demo
Optical sectioning methods
Pros&Cons
LMF TEAM
Dan / Jan
10:20 10:35 BREAK BREAK BREAK
10:35 13:00 SESSION 1
Theory & Demo
Optical sectioning methods
Pros&Cons
LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 2
Small Groups Discussion
What techniques might be good for me
(Own Projects)
LMF TEAM
Dan/Jan/Silke/Britta/Pete?
15:15 15:30 BREAK BREAK BREAK
15:30 16:30 SESSION 3 Course evaluation LMF TEAM

OFFICE DUTIES

- LMF Members available for users (19th Apr to 23rd Apr) -

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
9:30 12:30 - Silke Peter Peter Britta
12:30 13:30 L U N C H
13:30 18:00 Britta Dan Dan Silke -
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