Peter Evennett's Course Oct 09

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Peter Evennett's Course Oct 09

Friday 16th Oct

Setup - all lmf staff move stuff to galleria Equipment needed

  • Microscopes
  • Optical bench
  • Spinning disk gadget
  • Polarazing sheets
  • Diffraction Grids
  • Fluorescent samples
  • Fluorescent Beads (Prepare slides)
  • First day Handout for students
  • Light torches


DAY ONE (October 19th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:05 INTRODUCTION COURSE PRESENTATION
GROUP PRESENTATION
LMF TEAM
11:05 11:20 BREAK BREAK BREAK
11:20 13:00 SESSION 1
Theory & Practice
PRINCIPLES OF LIGHT MICROSCOPY
Historical aspects of microscopy
Introduction to lenses
Lens aberrations
Magnification
Numerical aperture
Microscope illumination
Conjugate planes
Koehler illumination
PETER EVENNETT
LMF TEAM
13:00 14:00 LUNCH LUNCH LUNCH
14:00 15:30 SESSION 2
Theory & Practice
PRINCIPLES OF LIGHT MICROSCOPY
Demo
PETER EVENNETT
LMF TEAM
15:30 15:45 BREAK BREAK BREAK
15:45 18:00 SESSION 3
Theory & Practice
PRINCIPLES OF LIGHT MICROSCOPY PETER EVENNETT
LMF TEAM

DAY TWO (October 20th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:45 SESSION 1
Theory & Practice
Resolution
Diffraction
PETER EVENNETT
LMF TEAM
10:45 11:00 BREAK COFFEE BREAK
11:00 13:00 DEMONSTRATION Abbe´s diffraction experiments
PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 16:00 SESSION 2
Theory & Practice
Bright Field
Dark Field
Phase Contrast microscopy
PETER EVENNETT
LMF TEAM
16:00 16:15 BREAK COFFEE BREAK
18:00 SESSION 2
Theory & Practice
Bright Field
Dark Field
Phase Contrast microscopy
PETER EVENNETT
LMF TEAM

DAY THREE (October 21th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 11:30 SESSION 1
Theory & Practice
Objective reading
Objective cleaning
PETER EVENNETT
LMF TEAM
11:30 11:45 BREAK BREAK BREAK
11:45 13:00 SESSION 2
Theory & Practice
Polarized light microscopy
Differential Interference Contrast (DIC)
PETER EVENNETT
LMF TEAM
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 3
Theory & Practice
Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy
PETER EVENNETT
LMF TEAM
15:15 15:30 BREAK BREAK BREAK
15:30 18:00 SESSION 4
Theory & Practice
Differential Interference Contrast (DIC)
Objective cleaning
Digital photomicrography
Introduction to Confocal Microscopy
PETER EVENNETT
LMF TEAM

DAY FOUR (October 22th)

FROM TO ACTIVITY TOPICS Responsible person(s) DEMO Activities DEMO materials
9:30 10:20 SESSION 1
Theory
Question session
Fundamentals concepts of fluorescence
LMF TEAM
Dan
- -
10:20 10:55 BREAK BREAK BREAK BREAK BREAK
10:55 13:15 SESSION 2
Theory & Practice
Fluorescence Microscopy LMF TEAM
Humberto
• Mercury lamp
• Lamp houses
• Spectra
• Filters
• Filter cubes
• Fluorescent-labeled samples
• Filter spectral charts of each microscope (laminated)
• Spare lamp houses
• Lamps
• Ocean optics equipment
• Different filters
• Four (4) different Filter-sets (cubes), with the appropriate descriptions
13:15 14:15 BREAK LUNCH LUNCH LUNCH LUNCH
14:15 15:45 SESSION 3
Theory & Practice
Imaging with CCD LMF TEAM
Britta
• CCD
• Cameras
• CCD chip
• Cameras
15:45 16:00 BREAK BREAK
16:00 18:00 SESSION 4
Practice
Imaging with CCD cameras using LMF systems LMF TEAM
Jan / Peter / Dan / Britta (*)
• Displaying
• Pixel size
• Look up table
• Binning
• Exposure time
• Saturation
• Signal to noise
• ROI
• Bit depth
• Auto map
• Auto contrast
• Microscopes (4)
• Sample slides

DAY FIVE (October 23th)

FROM TO ACTIVITY TOPICS Responsible person(s)
9:30 10:20 SESSION 1
Theory & Demo
Optical sectioning methods
Pros&Cons
LMF TEAM
Dan / Jan
10:20 10:35 BREAK BREAK BREAK
10:35 13:00 SESSION 1
Theory & Demo
Optical sectioning methods
Pros&Cons
LMF TEAM
Dan / Jan
13:00 14:00 BREAK LUNCH
14:00 15:15 SESSION 2
Theory & Demo
Basics of Quantitative Image Analysis
(Image Processing)
LMF TEAM
Dan
15:15 15:30 BREAK BREAK BREAK
15:30 16:30 SESSION 3 Course evaluation LMF TEAM

OFFICE DUTIES

- LMF Members available for users (19th Oct to 23rd Oct) -

FROM TO MONDAY TUESDAY WEDNESDAY THURSDAY FRIDAY
9:30 12:30 - Britta Peter Peter Silke
12:30 13:30 L U N C H
13:30 18:00 Britta Dan Silke (*)Silke
Humberto
-

(*) Silke and Humberto will be cleaning and reorganizing the Galeria

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