SD3 - Zeiss Spinning Disc

Suitable for fast live confocal imaging.

Directions

MPI-CBG LMF (MPI-CBG, 038B)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Zeiss - Axio Observer, Inverted stand, motorized with Definite Focus (hardware autofocus), 1.6x Optovar, Prior NanoScanZ, Märzhäuser xy scanning stage
objectives	Zeiss Plan-Apochromat 10x 0.45 Zeiss C-Apochromat 63x 1.2 W Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC Zeiss Alpha Plan-Apochromat 100x 1.46 Oil DIC Zeiss Plan-Apochromat 40x 1.3 Oil DIC Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC Zeiss LD C-Apochromat 40x 1.1 W Zeiss Plan-Apochromat 63x 1.4 Oil DIC
illumination	Transmitted Light (LED) Fluorescence (Metal Halide, HXP, 120W) Laser Diode 405 nm Laser DPSS 445 nm Laser DPSS 488 nm Laser DPSS 520nm Laser DPSS 561 nm Laser DPSS 639nm
detection	Spinning disc scan head Yokogawa CSU-X1 with two camera ports; pinhole radius: 25um, pinhole spacing: 250um; 3 DCs: (1) RTFT 405+488+561; (2) RQFT 405+488+568+647; (3) RTFT 457+514+647 Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50, (2) 458LP, (3) DBP 527/54 + 645/60, (4) 535/30, (5) 629/62, (6) BP 690/50; Roper evolve 515 EMCCD with manual EM filter holder
reflectors	Pos 1: empty for use with CSU Pos 2: FS49: Ex 365, BS 395, EM BP445/50 Pos 3: FS38HE: Ex BP470/40, BS 495, EM BP525/50 Pos 4: FS43HE: Ex BP550/25, BS 570, EM BP605/70 Pos 5: FS50: Ex BP640/30, BS 660, EM BP690/50 Pos 6: 80/20 mirror for FRAP
features	63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning. Pix spacing = 204.762 nm. Pinhole spacing is 250um and pinhole size is 50um diameter
software	Zeiss ZEN Blue 2.6
incubation	Cage incubator (T+CO2)
links	manual laser power values [ beam path] [ advanced system check]
inv.nr.	MOZG04081820000

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