SD4 - Andor Revolution WD Borealis Mosaic

Suitable for confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time.

Directions

MPI-CBG LMF (MPI-CBG, 036)

Booking

https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py

Details

microscope	Olympus - IX83, Inverted stand, motorized with ZDC (hardware autofocus and focus search), Prior NanoScanZ (400um travel range), Prior ProScanIII xy scanning stage
objectives	Olympus UPlan SApochromat 10x 0.4 Olympus UPlanSApo 30x 1,05 S Olympus UPLSAPO 40x 1.25 SIL Olympus UPLSAPO 60x 1.3 SIL Olympus UPlan SApochromat 100x 1.4 Oil Olympus UAPON 150x 1.45 TIRFM
illumination	Transmitted Light (LED) Fluorescence (LED) Laser Diode 405 nm Laser Diode 445 nm Laser DPSS 488 nm Laser DPSS 514 nm Laser DPSS 561 nm Laser DPSS 638nm Laser DPSS 726nm
detection	Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression; Camera 2 (Slave): Andor iXon 888 Ultra
reflectors	CSU-W scan head: 3 main DM: Tripple (for 445nm, 515nm, 640nm laser); Quad (405nm, 488nm, 561nm, 640nm); Penta (405nm, 488nm, 561nm, 640nm, 720nm) FW1 (Master): Pos(1) LP568, Pos(2) 525/50, Pos(3) 617/73, Pos(4) 607/36, Pos(5) 685/40, Pos(6) 785/62, Pos(7) pol, Pos(8) LP514, Pos(9) 542/27, Pos(10) 452/45 FW1 (Slave): Pos(1) 465/30, Pos(2) 541/30, Pos(3) 525/50, Pos(4) 479/40, Pos(5) pol, Pos(6) - Pos(10) empty reflectors in IX83 stand: DAPI set (Ex 377/50, DM 409, Em 447/60); GFP set (Ex 472/30, DM 495, Em 520/35); RFP set (Ex 562/40, DM 593, Em 624/40); CY5 set (Ex 628/40, DM 660, Em 692/40); Analyzer; DM for use with Mosaic
features
software	Andor iQ version 3.6; MetaMorph version 7.8 communication connections: COM1(Port1): IX83; COM3: CSU-WD; COM4: Mosaic shutter; COM6: prior XY stage; COM7: 16 channel CooLED; Device: Mosaic; Device1: Mosaic laser; DDA06/16 DAC board: Piezo, trigger box; ILE3082M-ILE3082S: ILE master, slave"Andor iQ version 3.6; MetaMorph version 7.8<br /> communication connections:<br />COM1(Port1): IX83; COM3: CSU-WD; COM4: Mosaic shutter; COM6: prior XY stage; COM7: 16 channel CooLED; <br />Device: Mosaic; Device1: Mosaic laser; DDA06/16 DAC board: Piezo, trigger box; ILE3082M-ILE3082S: ILE master, slave" cannot be used as a page name in this wiki.
incubation	Cage incubator (T+CO2)
links	[ manual] laser power values [ beam path] [ advanced system check]
inv.nr.

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