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We kindly invite you to an interactive [[M … We kindly invite you to an interactive [[Media:BioDIP-Spring-Seminar.pdf|seminar]] about Fluorescence Correlation Spectroscopy with talks and a follow-up discussion. This is the second seminar of our “Biopolis Dresden Imaging Platform (BioDIP) seminar series” focused on microscopy-driven research with talks given by:<br>
*Wolfgang Staroske: '''Fluorescence Cross-Correlation Spectroscopy: Disturbed protein interactions in rare diseases'''
*Mansi Gupta: '''FCS-based single molecule analysis of Fgf8 gradient formation in zebrafish'''
*Oliver Wüseke: '''Observing cytoplasmic interactions of centrosome proteins using FCS'''
Date: '''Wednesday, Mar 12th'''<br>
Time: '''15.00 - 17.00'''<br>
Location: '''MPI-CBG auditorium (small half)'''<br>
Host: '''BioDIP'''<br>
Proteins and their interactions are of central importance in biology, e.g. in every signalling cascade. Fluorescence (Cross-)Correlation Spectroscopy (F(C)CS) offers the possibility to investigate proteins and their interactions live, both in vitro and in vivo. To achieve that, F(C)CS analyzes the movement of fluorescent particles into and out of a focal volume at a confocal microscope and can deduce several parameters from this analysis. (1) FCS measures the mobility and concentration of a fluorescently tagged molecule of interest and thereby provides information about protein concentrations and associations. (2) FCCS uses two, fluorescently tagged molecules and yields quantitative data describing the interaction of these molecules (dissociation constant). In the BioDIP seminar we will span the range from the investigation of protein complexes in solution to measurements of protein interaction in the membrane of the living embryo. If you are curious to learn how you can use F(C)CS for probing the dynamics/interactions of your protein of interest, join us for the seminar and the discussion afterwards.the seminar and the discussion afterwards.
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