Ultramicroscope, CRTD

Suitable for 3D multicolor imaging of large transparent specimen.

Directions

Imaging@BIOTEC/CRTD (CRTD, -1.120)

Booking

https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c

Details

microscope	Olympus/MVX10, upright stand, motorized stage to move the sample holder in xyz inside the glass cuvette, 2 different dipping caps for the objective available
objectives	Olympus MVPLAPO 2x/0.5
illumination	Laser Fianium Supercontinuum SC400-4
detection	Andor Neo sCMOS camera Pixel 2560 x 2160 Pixel size 6.5 x 6.5 μm2
reflectors	7 excitation and emission filter: DAPI: ex. 405/40 ET, em. 460/50 ET Bandpass GFP: ex. 470/40 ET, em. 525/50 ET Bandpass YFP: ex. 500/20 ET, em. 535/30 ET Bandpass Cy3/DsRed/TRITC: ex. 545/30, em. 610/60 ET Bandpass mCherry/ TxRed: ex. 560/40, em. 630/75 ET Bandpass Alexa 610/Alexa 633/E2-Crimson: ex. 605/50, em. 670/50 ET Bandpass Cy7/Alexa 750/DyLight 750: ex. 710/75, em. 810/90 ET Bandpass
features	Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions."Two different cuvettes/sample holders/dipping caps are available, one for live imaging in water, one for fixed/cleared samples. There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction. The light sheet can be tuned in three dimensions: thickness and width of the sheet, overlap of the 2 sheets from opposite directions." cannot be used as a page name in this wiki.
software	LaVision BioTec ImSpector Software Windows 7, 64 bit"LaVision BioTec ImSpector Software<br /> Windows 7, 64 bit" cannot be used as a page name in this wiki.
incubation	Not available
links	[ manual] [ laser power values] [ beam path] [ advanced system check]
inv.nr.	0

---