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2014: GermanBioImaging Job Shadowing +	First Call for Job Shadowing Guests and Course Participants within the GerBI Educational Programme. Aim of the GerBI Educational Programme is to foster the exchange of know-how and to advance the level of expertise and professional competence within the Bioimaging Community, in particular among managers of Imaging Core Facilities. The ''Job Shadowing Programme'' promotes visits of Core Facility staff members to other Core Facilities to learn more about the operation procedures of such facilities and their teaching activities and/or about a specific imaging technique. The ''One Place for GerBI Programme'' enables interested scientists to take part in a training Course provided by Core Facilities or Research Groups outside their own institution. Please find more information and a list of "One Place for GerBI" courses and "Job Shadowing" hosts on http://www.germanbioimaging.org/wiki/index.php/1st_Call_for_Educational_Programme. If you like to apply, please fill in the Job Shadowing Guest and Course Participant form. German BioImaging will function as organisational platform to bring together course providers and applicants, Job Shadowing hosts and guests, and will also offer travel grants.
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April 21-22: Arivis Workshop +	2-day workshop on the visualization and analysis of multi-dimensional biological image data with arivis Vision4D. place: CRTD arivis: Christian Götze, Falko Löffler, Carola Bender & Tamara Manuelian Day 1 - 11 am: presentation of the new release arivis Vision4D 2.11 & basic operations Day 2 - 9 am: Hands-on sessions in groups
April 28-29: Andor Academy +	Andor Academy This free to attend scientific event is filled with cutting-edge scientific talks, interesting technical presentations and practical demo sessions. The academy will feature keynote speakers including Dr Jan Huisken (MPI-CBG) and Dr Jan Schmoranzer (Leibniz-Institut für Molekulare Pharmakologie). Please see the program here : http://www.andor.com/dresden.aspx
April 8, 2014: Label-free cell & tissue characterization by Raman spectroscopy +	We kindly invite you to the presentation of a novel tool for the label-free characterization of cells and tissue using Raman spectroscopy. Title: '''Raman spectroscopy- a novel method for label-free cell detection and characterization'''<br> Speaker: '''Rainer Gangnus (CellTool)'''<br> Time: '''1:30pm'''<br> Location: '''CRTD, Seminar room 2'''<br> Host: '''BioDIP / LMF CRTD/BIOTEC'''<br> Raman spectroscopy (RS) can be used to investigate molecular changes occurring during cell development and differentiation or being introduced by drugs and disease. The information retrieved is highly specific and the cells remain viable and unaffected.
August 18 - 29 2014: EMBO Course on Light Sheet Microscopy +	ABOUT THE PRACTICAL COURSE Light-sheet microscopy is an emerging technology that enables imaging of biological specimens with minimal photo-damage. It opens new avenues to study cell biological and developmental processes with unprecedented imaging speed. It also facilitates systems biology approaches by quantitative imaging of entire living systems. Light-sheet microscopy has only recently left the optical tables of technology developers and became more broadly available through commercial and open-access platforms. This EMBO Practical Course will comprehensively introduce the light sheet microscopy paradigm to the new generation of scientists. Visit the website: http://events.embo.org/14-lsm/
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December 11, 2013: Nanoscale dark field microscopy and Hyperspectral Imaging +	We will be hosting a seminar of a company called Cytoviva, which offers newly designed dark-field condenser offering high spatial resolution. Here is more info: http://www.cytoviva.com/products/microscopy-2/microscopy/<br> Date for talk: 11 December 2013<br> Time: 10am<br> Location: MPI-CBG, Galleria<br> Host: LMF MPI-CBG After the seminar there will be the possibility to test your samples on their setup in seminar room 1 in the MPI-CBG.
December 3, 2014: Seminar and hands-on microfluidics tools from "Biophysical Tools" +	Speaker: Dr. Claus Fütterer (Biophysical Tools, Leipzig, Germany) Title: Exploring liquid flow for biological and medical applications_ Novel instruments designed to talk to cells and tissues in time and space via liquids. Date: Wednesday, 3 December 2014 Time: 14:00 Place: Galleria Host: BioDIP
December 5, 2013: SPIM microscopy: biological applications and technology +	We kindly invite you to an interactive [[Media:2013-Dec05-BioDIP-Seminar.pdf|seminar]] about SPIM microscopy with talks given by experts in the field and a follow-up discussion. This will be the first seminar of our new “Biopolis Dresden Imaging Platform (BioDIP) seminar series” focused on microscopy-driven research with talks given by:<br> *Jan Huisken: '''SPIM technology'''<br> *Pavel Tomancak: '''SPIM data processing'''<br> *Jan Peychl: '''SPIM imaging from a core facility perspective'''<br> Date: Thursday, '''Dec 5th'''<br> Time: 15.00 - 17.00<br> Location: '''CRTD lecture hall''' (main hall, Auditorium left)<br> Host: Michael Brand / BioDIP<br> Selective/Single Plane Illumination Microscopy (SPIM) as a fast evolving and exiting new technique opens a new field due to deep penetration, fast acquisition of 3D data sets and long term time-lapse imaging (of live cells, tissues, developing embryos etc.) without photo damage. Jan Huisken and Pavel Tomancak have key expertise in SPIM technology and data processing and will share their insights during the talks. Jan Peychl will present his experiences with the Zeiss-SPIM-system from a core facility perspective. After the talks we would like to discuss over some wine and beer about the applications for your own research and the potential need for an additional SPIM system at the CRTD. Do not miss this seminar!
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February 16, 2015: "Engineering Fluorescence for Mircoscopy" seminar (Thermo Fisher) +	Engineering fluorescence for microscopy Speaker: Nick J Dolman PhD From: Research & Development Molecular Probes Labeling and Detection Technologies Thermo Fisher Scientific When: Friday, 16th January 2015 Time: 11:00 Where: Small Auditorium, MPI-CBG Fluorescence microscopy is one of the most commonly employed techniques in cell biology. Data from fluorescence imaging offers the benefit of both spatial and temporal resolution as well as multi-parametric analysis of cells in heterogenous populations. This technical seminar will provide an introduction to fluorescence, discussion of the properties of fluorescent dyes and proteins. Strategies for engineering fluorophores to optimize them for imaging applications will be described using recently developed technologies to illustrate these approaches. Considerations for labeling live and fixed cells will be presented with an emphasis on super-resolution microscopy.
February 3, 2015: "Applications of Raman Spectroscopy" +	Speaker: Dr. Karin Schütze (CellTool GmbH) Title: Photons on duty for health - Label-free and non-destructive investigation of cells and tissue When: 3pm, February 3, 2015 Where: CRTD, Seminar room 2 (east) Host: LMF/ BioDIP
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January 14, 2014: OMX imaging workshop: High resolution and fast widefield imaging +	Structuring your imaging: How the OMX system can help you to perform high resolution and fast widefield imaging '''Speakers:''' Dan White (GE Healthcare), Andreas Müller (PLID), Xin Liang (MPI-CBG), Uddipta Biswas (MTZ) '''Date:''' 14th January 2014 '''Time:''' 2pm - 4pm '''Place:''' MPI-CBG, Galleria The Optical Microscope eXperimental (OMX) system, which we have in-house, can be your instrument of choice when it comes to the acquisition of high resolution images (using Structured Illumination Microscopy = SIM) and fast multi-channel widefield imaging. This workshop intends to provide guidance, how the OMX system can help you with your imaging project. Dan White - application specialist for the OMX system - will introduce the OMX and principles of SIM. Furthermore, three users of the OMX system in the LMF will share their experiences with SIM and present their work. '''Here's the program:''' '''A. Introduction to the OMX system and SIM''' Dan White: ''Confocal busting resolution and contrast on the DeltaVision OMX - what can it do for your project?'' '''B. Application talks''' 1. Andreas Müller: ''Correlative light and electron microscopy for quantitative analysis of insulin granule biogenesis and turnover''<br> 2. Xin Liang: ''Structured illumination imaging of Drosophila sensory neurons''<br> 3. Uddipta Biswas: ''Using the OMX system for improved resolution of mammalian meiotic chromosomes, the synaptonemal complex and associated proteins''<br> '''C. Round table discussion'''<br>
January 14-22, 2014: Demo Andor spinning disk system with CSU-X1 and CSU-W1 +	We will have a latest-generation spinning disk demo system from Andor in the MPI-CBG. Built around a Nikon TiE stand it will feature two Yokogawa spinning disk scan heads (X1 and W1), each equipped with two cameras (iXon Ultra EMCCD and Neo CMOS).<br> '''The Details:''' '''Stand:''' Nikon TiE with motorized xy-stage and z-piezo (200µm travel range) '''Objective lenses:''' 10x and 20x air, 40x and 60x water, 40x and 100x oil, and 60x oil TIRF (however, not sure so far if system will be equipped with TIRF illuminator) '''Laser lines:''' 405, 445, 488, 514, 561, 640nm '''Spinning disk scan heads:''' (A) Yokogawa CSU-X1 (B) Yokogawa CSU-W1 (dual disk + bypass mode for widefield imaging) '''FRAPPA unit for bleaching and photoactivation''' '''Heating:''' Warner chamber<br> <br> If you're interested in testing or have specific requirements (e.g. emission filters) please tell us and we'll try to account for that.
January 27, 2015: seminar on "Microdissection" +	Speaker: Ulrich Sauer, Senior Application Consultant, Carl Zeiss Microscopy GmbH Title: Microdissection: Bridging the gap between microscopy and molecular analyses Date: Tuesday, 27 January 2015 Time: 10:00 AM Place: Seminar Room 2
June 18, 2014: Leica Super-Resolution STED/GSD seminar +	You are interested in super-resolution light microscopy? Then don't miss the following seminar given by Leica: Title: '''„New Dimensions in Super-Resolution Microscopy” Leica TCS SP8 STED 3X and Leica SR GSD 3D'''<br> Speaker: '''Dr. Olga Lévai, Leica Microsystems'''<br> Date: '''Wednesday, Jun 18th'''<br> Time: '''15:00 - 16:30 h'''<br> Location: '''CRTD, seminar room 2nd floor'''<br> Host: '''LMF CRTD/BIOTEC'''<br> Everybody is welcome!
June 23-25: BioDIP is visited by Gertrude Bunt and Sabine Haxelmans +	From June 23-25 the BioDIP hosts two guests, Gertrude Bunt (Göttingen) and Sabine Haxelmans (Freiburg) The guests will visit all BioDIP Light Microscopy Facilities and meet representatives of the administration of the BioDIP institutions.
June 24, 2014: BioDIP Summer Seminar CARS Microscopy - a new label free imaging technique +	We kindly invite you to an interactive [[Media:BioDIP-Summer-Seminar.png|seminar]] about CARS Microscopy with talks and a follow-up discussion. This is the third seminar of our “Biopolis Dresden Imaging Platform (BioDIP) seminar series” focused on microscopy-driven research with talks given by:<br> *PD Dr. Gerald Steiner / Roberta Galli (Clinical Sensoring and Monitoring): '''Cells and tissue in the spotlight: label-free optical analysis in Dresden''' *Prof. Dr. Matthias Kirsch / Dr. Ortrud Uckermann (Department of Neurosurgery): '''The optical fingerprint of brain tumors''' *Prof. Dr. Matthias Kirsch / Robert Later (Department of Neurosurgery and CRTD): '''CARS imaging of the regenerating axolotl spinal cord''' Date: '''Tuesday, Jun 24th'''<br> Time: '''15.00 - 17.00'''<br> Location: '''CRTD auditorium (left half)'''<br> Host: '''BioDIP'''<br> Resolving biological processes often relies on increasing the contrast by staining with e.g. fluorescent dyes. This labeling step can perturb the specimen and is often not desired in e.g. investigations in vivo.<br> Vibrational spectroscopy, such as Raman and infrared spectroscopy, is a label free technique that provides direct molecular and structural information. The spectra of these complementary methods are hence referred to as molecular fingerprint. Infrared spectroscopy imaging and Coherent anti Stokes Raman spectroscopy (CARS) are relatively new techniques for a very fast and sensitive label-free microscopy of cells and tissues.<br> We will present applications of these fast, label-free and in vivo techniques, among others for studying spinal cord regeneration in several animal models (rat, axolotl) and for observing tumor cells both in vivo and in cell culture. So if you are interested in a fast, label-free and in vivo imaging technique, do not miss the seminar.
June 24, 2014: BioDIP summer seminar "CARS Microscopy: a new label-free imaging technique" +	CARS Microscopy: a new label-free imaging technique Speakers: PD Dr. Gerald Steiner / Roberta Galli: Cells and tissue in the spotlight: label-free optical analysis (Clinical Sensoring and Monitoring) Prof. Dr. Matthias Kirsch / Dr. Ortrud Uckermann: The optical fingerprint of brain tumors (Department of Neurosurgery) Prof. Dr. Matthias Kirsch / Robert Later: CARS imaging of the regenerating axolotl spinal cord (Department of Neurosurgery and CRTD) Date: 24th of June 2014 Time: 3-5 pm Location: CRTD, Auditorium (left half) Host: BioDIP Resolving biological processes often relies on increasing the contrast by staining with e.g. fluorescent dyes. This labeling step can perturb the specimen and is often not desired e.g. in investigations in vivo. Vibrational spectroscopy, such as Raman and infrared spectroscopy, is a label free technique that provides direct molecular and structural information. The spectra of these complementary methods are hence referred to as molecular fingerprint. Infrared spectroscopy imaging and Coherent anti Stokes Raman spectroscopy (CARS) are relatively new techniques for a very fast and sensitive label-free microscopy of cells and tissues. We will present applications of these fast, label-free and in vivo techniques, among others for studying spinal cord regeneration in several animal models (rat, axolotl) and for observing tumor cells both in vivo and in cell culture.
June 25, 2014: STED "hands-on" demo by Abberior +	This is the possibility to check own samples with a STED microscope (resolution below 200nm). Abberior is bringing a multicolot STED system on a truck, that is ready to be used! Location: parking lot of the MPI-CBG Date: 25th of June 2014
June 30: BioDIP Seminar "Clearing Techniques" +	BioDIP Summer Seminar "Less scatter more image: tissue clearing techniques" Speakers: Uwe Schröer (LaVision BioTec): "Sample Preparation of Thick Tissues for Light Sheet Microscopy" Olaf Selchow (Carl Zeiss Microscopy): "Imaging Optically Cleared Specimen with Light Sheet Fluorescence Microscopy" Date: June 30, 2015 Time: 2-4pm Location: CRTD, Seminar room 2 Tissue clearing allows imaging deep into large biological samples such as tissue sections, brains, embryos, organs or spheroids. The enhanced optical penetration depth can be used to image fluorescently labeled structures within large tissue samples such as whole mouse brains with high resolution. The focus of this seminar will be to show different methods of tissue clearing and how cleared samples are imaged best.
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March 10, 2014: Seminar & hands on demo next week: Zeiss slide Scanner Axio Scan.Z1 +	We kindly invite you to the seminar with hands on demo of the new Zeiss Slide Scanner Axio Scan.Z1. Together with the ZEN Software it is a new Whole Slide Imaging Platform for biomedical research. The system is highly flexible, offers open software interfaces and is especially recommended for multi-user environments like imaging facilities. Please find more infos at http://www.zeiss.com/axioscan<br> You are very welcome to test the instrument with your own slides next week! Please get in touch with us via email for reserving a demo slot.<br> There will be an '''introductory talk''' given:<br> Title: Axio Scan.Z1 - The new Zeiss Slide Scanner<br> Speaker: Thomas Betz (Zeiss)<br> Date for talk: '''10th March 2014'''<br> Time: 1pm<br> Location: CRTD, Seminar room 3<br> The '''DEMO''' will take place:<br> Date for demo: '''11 - 13 March 2014'''<br> Location: BIOTEC, Room 225<br> Host: BioDIP / LMF CRTD/BIOTEC<br>
March 11, 2015: Quantitative Phase Imaging +	Speaker: Arnaud Rehel (PHASICS) Title: Quantitative phase imaging for biology Date: Wednesday, 11 March 2015 Time: 10:00 Place: Seminar Room 4th floor Host: Moritz Kreysing & LMF team Seminar and Demo. The demo takes place in meeting room 301 South (MPI-CBG).
March 12, 2014: BioDIP Spring Seminar: Fluorescence Correlation Spectroscopy +	We kindly invite you to an interactive [[Media:BioDIP-Spring-Seminar.pdf‎|seminar]] about Fluorescence Correlation Spectroscopy with talks and a follow-up discussion. This is the second seminar of our “Biopolis Dresden Imaging Platform (BioDIP) seminar series” focused on microscopy-driven research with talks given by:<br> *Wolfgang Staroske: '''Fluorescence Cross-Correlation Spectroscopy: Disturbed protein interactions in rare diseases''' *Mansi Gupta: '''FCS-based single molecule analysis of Fgf8 gradient formation in zebrafish''' *Oliver Wüseke: '''Observing cytoplasmic interactions of centrosome proteins using FCS''' Date: '''Wednesday, Mar 12th'''<br> Time: '''15.00 - 17.00'''<br> Location: '''MPI-CBG auditorium (small half)'''<br> Host: '''BioDIP'''<br> Proteins and their interactions are of central importance in biology, e.g. in every signalling cascade. Fluorescence (Cross-)Correlation Spectroscopy (F(C)CS) offers the possibility to investigate proteins and their interactions live, both in vitro and in vivo. To achieve that, F(C)CS analyzes the movement of fluorescent particles into and out of a focal volume at a confocal microscope and can deduce several parameters from this analysis. (1) FCS measures the mobility and concentration of a fluorescently tagged molecule of interest and thereby provides information about protein concentrations and associations. (2) FCCS uses two, fluorescently tagged molecules and yields quantitative data describing the interaction of these molecules (dissociation constant). In the BioDIP seminar we will span the range from the investigation of protein complexes in solution to measurements of protein interaction in the membrane of the living embryo. If you are curious to learn how you can use F(C)CS for probing the dynamics/interactions of your protein of interest, join us for the seminar and the discussion afterwards.
March 3, 2015: BioDIP Seminar "Multiphoton Microscopy" +	BioDIP Winter Seminar on Multiphoton Microscopy: Imaging deep within tissues and live animals Speakers: Sebastian Bundschuh "Introduction to Multiphoton Microscopy" Michael Gerlach "In vivo imaging of islets of Langerhans adaptation to pregnancy in an anterior chamber transplantation model" Jan Dudeck: "Intravital microscopy to monitor inflammation induced innate immune networking" Date: March 3, 2015 Time: 3-5pm Location: CRTD, Auditorium (right half)
March 4, 2014: Refractometer Demo +	We have the pleasure to invite you all for the demonstration of an advanced refractometer, the J457 from Rudolf Research Analytical, which might well suit the needs of the community of microscopists of the whole campus. Indeed, this machine has among all the other characteristics a measurement range of refractive index that goes from 1.26 to 1.72, it's highly accurate, temperature control (10-110°C), and can measure through solid matter too (e.g. animal tissues).<br> '''Seminar:''' 11:15 MPI-CBG Galleria<br> '''Practical Part:''' Available for testing for one week in MPI-CBG LMF wet lab (room 036)<br> It is very important for us all to understand the real capabilities of the machine. And since Mr. Schmidt, who will come for the demonstration, would be available to test real samples the on 4th of March, please bring your media, specimens, and challenging tissues. In the case you would like to measure solid materials, just consider that the diameter of the sample should be less than 10 mm, and that hard samples like glass have to be polished.
May 11, 2015: "microDimensions seminar" +	microDimensions - beyond the visual limits Speaker: Dr. Martin Groher, CEO microDimensions Date: May 11, 2015 Time: 2pm Place: CRTD, seminar room 1 Host: Biopolis Dresden Imaging Platform (BioDIP) The LMF BIOTEC / CRTD host a Zeiss slide scanner (Axio Scan.Z1) optimally suited to create virtual slides e.g. as basis for 3d reconstructions. A concise system overview will be given by Hella Hartmann.

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