Light Microscopy for You
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(add link for new user checklist and lots of content) |
m (moved BasicLMFIntroCourse to Light Microscopy for You: give the page a proper name) |
Revision as of 11:51, 20 September 2010
1 slide per point
- What is a Microscope - Provdides Magnification and more importantly Resolution.
- Basic Ray Optics of a Lens.
- refraction
- Conjugate planes
- Single Lens has 2 focal planes FFP and BFP
- little microscope
- What is confocal
- Resolution
- optical resolution - Point Spread Function / Airy Disk
- Pixel Spacing (size?) Physical CCD dexels vs Picture Elements vs point scanning
- Numerical Aperture vs Magnification : Abbe/Rayleigh Resolution d = lambda / 2NA
- What is contrast? (No light = no image; no contrast = no resolution)
- Fluorescence and Stokes Shift
- Spectrum databases / viewers for planning expt.
- Fluorescence and Stokes Shift
- Detectors: CCD vs. PMT Widefield vs point detection
- Digitization
- Detector Saturation
- Objective language / reading
- Coverslip thickness is part of the objective design (#1.5 0.17 mm +- 0.01)
- Lens Immersion media
- old oil, wrong oil, wrong collar setting
- Lens cleaning
- safety ; mercury lamps, lasers (jewelry, interlocks)
- Project Planning / Workflow
- Admin intro
- New User 30 min new user checklist