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Images acquired with our equipment

Effect of the CLSM pinhole

overview images

  • Typical overview image of a tissue sample region. Pinhole diameter decreases from 8 Airy units to 1 Airy unit.

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm + 594 nm excitation, 505-580 nm + LP610 nm detection

XY

  • High resolution image, acquired with decreasing pinhole diameter from 8 Airy units to 0.6 Airy units.

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 594 nm excitation, LP610 nm detection

XYZ

  • One region of the sample was imaged with decreasing pinhole diameter, starting from 8 airy units (first image) down to 1 airy units (last image).

  • Invitrogen FluoCells(r) #4, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488 nm excitation, 505-580 nm detection, 0.2 µm Z-stepsize, Bitplane Imaris

Cleaning an objective makes sense

  • Invitrogen FluoCells(r) #6, Zeiss LSM 510, Plan-Apochromat 63x/1.4, 488nm excitation, 505-550nm detection, pinhole @98µm

Photos

Peter Evennett

"Principles of Light Microscopy" course 2007

PhD Course

2005

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