LSM 510, inverse, BIOTEC
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{{Equipment | {{Equipment | ||
− | | system-number | + | |system-number=BIO10 |
− | | system-name | + | |system-name=LSM 510, inverse, BIOTEC |
− | | category | + | |type=Not listed |
− | + | |category=Laser Scanning Confocal | |
− | | facility | + | |facility=Imaging@BIOTEC/CRTD |
− | | | + | |building=BIOTEC |
− | | | + | |room=227 |
− | | | + | |Facility-logo=Imaging@Biotec.jpg |
− | | description | + | |Institute-logo=Biotec logo.png |
− | | | + | |description=Our [[Zeiss LSM 510]] [[confocal]] laser scanning microscope is designed for routine work and dedicated for high resolution imaging in XYZ. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. |
− | | | + | |applications=- confocal scanning microscopy |
− | | detection | + | |image=Bioz01.jpg |
− | | features | + | |stand-name=Zeiss - Axiovert 200M |
+ | |stand=Inverted | ||
+ | |microscope=motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
+ | |obj1=Zeiss EC Plan-Neofluar 10x 0.3 | ||
+ | |obj2=Zeiss EC Plan-Neofluar 20x 0.5 | ||
+ | |obj3=Zeiss C-Apochromat 40x 1.2 W | ||
+ | |obj4=Zeiss C-Apochromat 63x 1.2 W | ||
+ | |obj5=Zeiss Plan-Apochromat 63x 1.4 Oil | ||
+ | |obj6=Zeiss alpha Plan-Fluar 100x 1.45 Oil | ||
+ | |ill1=Transmitted Light (Halogen) | ||
+ | |ill2=Fluorescence (HBO) | ||
+ | |ill3=Laser Diode 405 nm | ||
+ | |ill4=Laser Argon Multiline 458, 477, 488, 514 nm | ||
+ | |ill5=Laser HeNe 543 nm | ||
+ | |ill6=Laser HeNe 633 nm | ||
+ | |detection=point scanner, 2 confocal PMTs, 1 META detector, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
+ | |reflectors=DAPI : EX 365/12 ; BS 395 ; EM LP 397<br>FITC : EX 470/40 ; BS 510 ; EM LP 515<br>TRITC : EX 546/12 ; BS 580 ; EM LP 590 LP | ||
+ | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
+ | |software=Zeiss AIM | ||
+ | |incubation=Stage incubator (T) | ||
+ | |link0=http://www.biotec.tu-dresden.de/fileadmin/technology/imaging/LSM_510_-_manual.pdf | ||
+ | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_:_how_to_measure_and_values | ||
+ | |link2=- | ||
+ | |inv=121528 | ||
+ | |objectives=10x/0.3<br>20x/0.5<br>40x/0.95 corr<br>40x/1.2 W corr<br>100x/1.4 Oil DIC | ||
+ | |illumination=HBO lamp<br>Halogen lamp<br>405 diode laser<br>458, 477(optional), 488, 514 argon laser<br>543 HeNe laser<br>633 HeNe laser | ||
}} | }} |
Latest revision as of 13:25, 23 October 2013
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
microscope | Zeiss - Axiovert 200M, Inverted stand, motorized XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC | ||
objectives | |||
illumination | |||
detection | point scanner, 2 confocal PMTs, 1 META detector, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
reflectors | DAPI : EX 365/12 ; BS 395 ; EM LP 397 FITC : EX 470/40 ; BS 510 ; EM LP 515 TRITC : EX 546/12 ; BS 580 ; EM LP 590 LP | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
software | Zeiss AIM | ||
incubation | Stage incubator (T) | ||
links |
| ||
inv.nr. | 121528 |