MTZ02 - Leica TCS SP5
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{{Equipment | {{Equipment | ||
− | | system-number | + | |system-number=MTZ02 |
− | | system-name | + | |system-name=Leica TCS SP5 |
− | | category | + | |type=Microscope |
− | + | |category=Laser Scanning Confocal | |
− | | facility | + | |facility=MTZ Imaging |
− | + | |building=MTZ | |
− | | building | + | |room=B20.001C |
− | | room | + | |Facility-logo=Logo mtz 3.png |
− | | description | + | |Institute-logo=Logo Medizin.png |
− | | microscope | + | |description=This [[Leica TCS SP5]] [[confocal]] microscope is specialized for Life Cell Imaging, FRET and FRAP. Modules for time-series and bleaching experiments are available. |
− | | | + | |applications=fixed cell imaging, life cell imaging, FRET and FRAP, multi position acquisition, [[tile scan]], routine dye combination as well as exotic ones. Optimal for EGFP/mCherry or CFP/YFP/mCherry |
− | | | + | |image=MTZ02.jpg |
− | | detection | + | |stand-name=Leica - DMI6000 |
− | | reflectors | + | |stand=Inverted |
− | | features | + | |microscope=inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC |
− | | incubation | + | |obj1=Leica HC PL APO 10x 0.4 |
+ | |obj2=Leica HC PL APO 20x 0.7 | ||
+ | |obj3=Leica HCX PL APO 40x 1.25-0.75 Oil | ||
+ | |obj4=Leica HCX PL APO 63x 1.4-0.6 Oil | ||
+ | |obj5=Leica HCX PL APO 63x 1.2 W | ||
+ | |ill1=Transmitted Light (Halogen) | ||
+ | |ill2=Fluorescence (HBO) | ||
+ | |ill3=Laser Diode 405 nm | ||
+ | |ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | ||
+ | |ill5=Laser HeNe 543 nm | ||
+ | |ill6=Laser HeNe 594 nm | ||
+ | |ill7=Laser HeNe 633 nm | ||
+ | |detection=point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole<br>optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) | ||
+ | |reflectors=DAPI (EX 360/40, EM LP425)<br>GFP (EX 470/40, EM LP515)<br>RFP (EX 538/45, EM LP590) | ||
+ | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro | ||
+ | |incubation=Cage incubator (T) | ||
+ | |link1=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNumJpfihZLcaw | ||
+ | |link2=https://spreadsheets.google.com/pub?key=pMZJXCgd5HNsytK4WLaxaWQ | ||
+ | |inv=- | ||
+ | |facility_image=logo-mtzimaging.jpg | ||
+ | |objectives=10x/0.4 dry<br>20x/0.7 dry<br>40x/1.25 Oil<br>63x/1.4 Oil Apo<br>63x/1.2 W Apo | ||
+ | |illumination=Halogen<br>HBO<br>405 nm Diode laser<br>458, 477, 488, 496, 514 nm Argon laser<br>543 nm HeNe laser<br>594 nm HeNe laser<br>633 nm HeNe laser | ||
}} | }} | ||
+ | [[Category:Wide-Field|i]] |
Latest revision as of 16:52, 11 November 2013
[edit] Directions
[edit] Booking
https://cgc.med.tu-dresden.de/phpScheduleIt
[edit] Details
microscope | Leica - DMI6000, Inverted stand, inverted stand, motorized XY stage, motorized Z-drive, fast Z-stage, fluorescence, transmitted light with motorized DIC | ||
objectives | |||
illumination | |||
detection | point scanner, 3 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, prism and spectral sliders for detection range selection, central pinhole optional wide-field detection via color camera, CCD Leica DFC 500 (1360x1024 up to 4080x3072, 6.45x6.45µm pixel) | ||
reflectors | DAPI (EX 360/40, EM LP425) GFP (EX 470/40, EM LP515) RFP (EX 538/45, EM LP590) | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, fast XZ-scans, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, tile scan, multi position scan, lambda scan, FRET macro, FRAP macro | ||
software | |||
incubation | Cage incubator (T) | ||
links |
| ||
inv.nr. | - |