CZ2 - Zeiss LSM UV
From BioDIP
(Difference between revisions)
(34 intermediate revisions by 5 users not shown) | |||
Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
− | | system-number | + | |system-number=MPI-CZ2 |
− | | system-name | + | |system-name=Zeiss LSM UV |
− | | category | + | |type=Not listed |
− | | | + | |category=Laser Scanning Confocal |
− | | | + | |facility=MPI-CBG LMF |
− | + | |building=MPI-CBG | |
− | | | + | |room=038A |
− | | | + | |Facility-logo=MPI-CBG-Lmf-logo-big.png |
− | + | |Institute-logo=Logo MPI-CBG.jpg | |
− | | description | + | |description=Single photon [[Zeiss LSM 510]] [[confocal]] system with three PMTs and transmitted light detector. Inverted setup with UV laser. |
− | | applications | + | |applications=for routine dye combinations as well as special stainings that require UV excitation, i.e. lipid dyes. |
− | | | + | |image=CZ2 - Zeiss LSM UV.jpg |
− | | | + | |stand-name=Zeiss - Axiovert 200M |
− | | | + | |stand=Inverted |
− | | detection | + | |microscope=manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC. Axiovert 200 M 1005-820 SN: 000181 Baujahr 2001. |
− | | reflectors | + | |obj1=Zeiss Plan-Apochromat 10x 0.45 |
− | | features | + | |obj2=Zeiss Plan-Neofluar 25x 0.8 Oil/Gly/Water |
− | | incubation | + | |obj3=Zeiss Plan Neofluar 40x 1.3 Oil |
− | | link1 | + | |obj4=Zeiss Plan-Neofluar 100x 1.3 Oil |
− | | link2 | + | |obj5=Zeiss Plan-Apochromat 63x 1.4 Oil |
− | | inv | + | |ill1=Transmitted Light (Halogen) |
+ | |ill2=Fluorescence (Metal Halide, X-Cite, 120W) | ||
+ | |ill3=Laser UV 351, 364 nm | ||
+ | |ill4=Laser Argon Multiline 458, 477, 488, 514 nm | ||
+ | |ill5=Laser HeNe 543 nm | ||
+ | |ill6=Laser HeNe 633 nm | ||
+ | |detection=point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
+ | |reflectors=EX 359-371, EM LP397 (Zeiss FS01)<br>EX 449-498, EM LP520 (Zeiss FS09)<br>EX 540-551, EM LP590 (Zeiss FS15) | ||
+ | |features=simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation | ||
+ | |software=- | ||
+ | |incubation=Stage incubator (T) + objective heater | ||
+ | |link0=https://ifn.mpi-cbg.de/wiki/MPI-CZ2_-_Zeiss_LSM_UV_-_startup_%26_shutdown | ||
+ | |link1=http://spreadsheets.google.com/pub?key=pFACvabR4mSk0c_q3sEgGIw | ||
+ | |link2=http://spreadsheets.google.com/pub?key=pMZJXCgd5HNsP92avQu4iGA | ||
+ | |inv=2002-01425 SIP: 71648 10.1.34.217 | ||
}} | }} |
Latest revision as of 15:42, 3 February 2016
[edit] Directions
[edit] Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
[edit] Details
microscope | Zeiss - Axiovert 200M, Inverted stand, manual XY stage, motorized Z-drive, fluorescence, transmitted light with manual DIC. Axiovert 200 M 1005-820 SN: 000181 Baujahr 2001. | ||
objectives | |||
illumination | |||
detection | point scanner, 3 confocal PMTs, T-PMT, dichromatic mirrors for excitation/emission splitting, emission filters for detection range selection, pinhole for each detector | ||
reflectors | EX 359-371, EM LP397 (Zeiss FS01) EX 449-498, EM LP520 (Zeiss FS09) EX 540-551, EM LP590 (Zeiss FS15) | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, time-series, averaging, summarizing, scan zoom and rotation | ||
software | - | ||
incubation | Stage incubator (T) + objective heater | ||
links |
| ||
inv.nr. | 2002-01425 SIP: 71648 10.1.34.217 |