Live Cell/TIRF AF6000, BIOTEC
From BioDIP
(Difference between revisions)
(13 intermediate revisions by 3 users not shown) | |||
Line 1: | Line 1: | ||
{{Equipment | {{Equipment | ||
− | |system-number= | + | |system-number=BIO08 |
− | |system-name= | + | |system-name=Live Cell/TIRF AF6000, BIOTEC |
|type=Microscope | |type=Microscope | ||
|category=Total Internal Reflection | |category=Total Internal Reflection | ||
− | |facility=Imaging@ | + | |facility=Imaging@BIOTEC/CRTD |
− | |building= | + | |building=BIOTEC |
|room=226 | |room=226 | ||
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
− | |Institute-logo= | + | |Institute-logo=Biotec logo.png |
|description=Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal. | |description=Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal. | ||
|applications=- | |applications=- | ||
|image=Bioz06 - Leica AFLX6000 TIRF.jpg | |image=Bioz06 - Leica AFLX6000 TIRF.jpg | ||
− | |stand-name=Leica - | + | |stand-name=Leica - DMI6000 |
|stand=Inverted | |stand=Inverted | ||
|microscope=motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF | |microscope=motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF | ||
Line 18: | Line 18: | ||
|obj2=Leica HC PL APO 20x 0.7 | |obj2=Leica HC PL APO 20x 0.7 | ||
|obj3=Leica HCX PL APO 40x 0.85 | |obj3=Leica HCX PL APO 40x 0.85 | ||
− | |obj4=Leica | + | |obj4=Leica HC PL APO 100x 1.46 Oil |
|ill1=Laser Diode 405 nm | |ill1=Laser Diode 405 nm | ||
|ill2=Laser DPSS 488 nm | |ill2=Laser DPSS 488 nm | ||
Line 25: | Line 25: | ||
|ill5=Fluorescence (Metal Halide, HXP, 120W) | |ill5=Fluorescence (Metal Halide, HXP, 120W) | ||
|ill6=Transmitted Light (Halogen) | |ill6=Transmitted Light (Halogen) | ||
− | |detection=Andor EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD | + | |detection=Andor iXON 897 EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD |
|reflectors=FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>CY5 : EX 635/10 ; BS 650 ; EM BP 720/60<br>Quad Laser Line<br>VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65<br>RFP : EX 552/24 ; BS 570 ; EX 605/65 | |reflectors=FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30<br>CY5 : EX 635/10 ; BS 650 ; EM BP 720/60<br>Quad Laser Line<br>VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65<br>RFP : EX 552/24 ; BS 570 ; EX 605/65 | ||
|features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD) | |features=sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD) | ||
− | |software= | + | |software=LAS AF |
|incubation=Cage incubator (T+CO2) | |incubation=Cage incubator (T+CO2) | ||
− | |link1=- | + | |link1=https://www.biotec.tu-dresden.de/lmf-wiki/index.php/Laser_power_AF6000_TIRF |
|link2=- | |link2=- | ||
− | |inv= | + | |inv=71925 |
|facility_image=Imaging@Biotec.jpg | |facility_image=Imaging@Biotec.jpg | ||
|logo_image=LOGO_CRTD.jpg | |logo_image=LOGO_CRTD.jpg |
Latest revision as of 13:44, 17 October 2014
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
microscope | Leica - DMI6000, Inverted stand, motorized XY stage, Super Z-stage, fluorescence, transmitted light with automatic DIC, TIRF | ||
objectives | |||
illumination | |||
detection | Andor iXON 897 EMCCD (512*512, 16µm pixels), Leica DFC (1392*1040, 6,45µm pixels), reflectors for fluorescence and TIRF, external emission filter wheel for rapid acquisition with Andor EMCCD | ||
reflectors | FITC (L5) : EX 480/40 ; BS 505 ; EM 527/30 CY5 : EX 635/10 ; BS 650 ; EM BP 720/60 Quad Laser Line VBG : EX 490/20 and 422/44 and 552/24 ; BS 435 and 505 and 550 ; EM 465/45 and 545/55 and 610/65 RFP : EX 552/24 ; BS 570 ; EX 605/65 | ||
features | sequential imaging, Z stacks, time-series, advanced time-series, multiple position imaging, fast acquisition (with Andor EMCCD) | ||
software | LAS AF | ||
incubation | Cage incubator (T+CO2) | ||
links |
| ||
inv.nr. | 71925 |