SP5 II, upright, CRTD
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|category=Laser Scanning Confocal | |category=Laser Scanning Confocal | ||
|facility=Imaging@BIOTEC/CRTD | |facility=Imaging@BIOTEC/CRTD | ||
− | |building= | + | |building=BIOTEC |
− | |room= | + | |room=220 |
|Facility-logo=Imaging@Biotec.jpg | |Facility-logo=Imaging@Biotec.jpg | ||
− | |Institute-logo= | + | |Institute-logo=Biotec logo.png |
|description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | |description=Our [[Leica TCS SP5]] II [[confocal]] setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with. | ||
|applications=- | |applications=- | ||
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|stand-name=Leica - DM6000 | |stand-name=Leica - DM6000 | ||
|stand=Upright | |stand=Upright | ||
− | |microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | + | |microscope=motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC |
|obj1=Leica HC PL APO CS 10x 0.4 | |obj1=Leica HC PL APO CS 10x 0.4 | ||
|obj2=Leica HC PL APO CS 20x 0.7 | |obj2=Leica HC PL APO CS 20x 0.7 | ||
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|obj7=Leica HCX APO L 40x 0.8 W | |obj7=Leica HCX APO L 40x 0.8 W | ||
|ill1=Transmitted Light (Halogen) | |ill1=Transmitted Light (Halogen) | ||
− | |ill2=Fluorescence (HXP) | + | |ill2=Fluorescence (Metal Halide, HXP, 120W) |
|ill3=Laser Diode 405 nm | |ill3=Laser Diode 405 nm | ||
|ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm | |ill4=Laser Argon Multiline 458, 477, 488, 496, 514 nm |
Latest revision as of 11:05, 2 November 2017
[edit] Directions
[edit] Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc142df4c59eef5c
[edit] Details
microscope | Leica - DM6000, Upright stand, motorized XYZ stage, Z-piezo stage, fluorescence, transmitted light with automatic DIC | ||
objectives | |||
illumination | |||
detection | point scanner, 5 confocal PMTs, T-PMT, AOBS for excitation/emission splitting, SP detector for detection range selection, common pinhole | ||
reflectors | DAPI (A) : EX 360/40 ; BS 400 ; EM LP 425 GFP (L5) : EX 480/20 ; BS 495 ; EM 535/40 | ||
features | simultaneous imaging, sequential imaging, line scan, point scan, Z stacks, multi-position imaging, time-series, advanced time-series, averaging, summarizing, scan zoom and rotation, FRAP | ||
software | LAS AF | ||
incubation | Cage incubator (T+CO2) | ||
links |
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inv.nr. | 71927 |