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| <br> | | <br> |
| *FS Analysator for DIC and Transmission | | *FS Analysator for DIC and Transmission |
− | |features=*sequential imaging | + | |features=<br> |
| + | *sequential imaging |
| *Brightfield and Fluorescence overlay | | *Brightfield and Fluorescence overlay |
| *timelapse | | *timelapse |
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| *tile scans | | *tile scans |
| *multi position experiments | | *multi position experiments |
− | *complex experiment via Experiment Designer | + | *complex experiments via Experiment Designer |
| *optical sectioning | | *optical sectioning |
| |software=ZEN Blue 2012 | | |software=ZEN Blue 2012 |
Revision as of 09:58, 3 August 2016
Suitable for
- fixed samples
- fluorescently labeled samples (structured illumination possible)
- histologically fixed samples (no strucutred illumination)
- big samples"<br />
- fixed samples
- fluorescently labeled samples (structured illumination possible)
- histologically fixed samples (no strucutred illumination)
- big samples" cannot be used as a page name in this wiki..
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Directions
Booking
https://techpool.biotec.tu-dresden.de/schedule/schedule.php?scheduleid=sc14ffd3aa3e44c3
Details
microscope
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Zeiss - Axio Imager.M2, Upright stand, motorized XY stage (Märzhäuser SMC2009), motorized z-drive, fluorescence, transmitted light with DIC, Darkfield b/w epifluorescence acquisition, Optical Sectioning (ApoTome2)
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objectives
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- Zeiss EC Plan Neofluar 5x 0.16
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss Plan-Apochromat 20x 0.8
- Zeiss Plan-Apochromat 40x 0,95 korr.
- Zeiss EC Plan-Neofluar 100x 1.3 Oil
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illumination
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- Fluorescence (Metal Halide, HXP, 120W)
- Transmitted Light (Halogen)
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detection
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- Axiocam MRm rev.3 (1388*1040, 6,45*6,45µm)
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reflectors
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- FS 49 (DAPI) : EX BP G365; BS 395; EM BP 445/50
- FS 38 HE (GFP) : EX BP 470/40; BS 495; EM BP 525/50
- FS 20 (Rhodamine) : EX BP 546/12; BS 560; BP 575-640
- FS 50 (Cy5) : EX BP 640/30 ; BS 660 ; EM 690/50
- FS Analysator for DIC and Transmission
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features
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- sequential imaging
- Brightfield and Fluorescence overlay
- timelapse
- z-stack
- tile scans
- multi position experiments
- complex experiments via Experiment Designer
- optical sectioning"<br />
- sequential imaging
- Brightfield and Fluorescence overlay
- timelapse
- z-stack
- tile scans
- multi position experiments
- complex experiments via Experiment Designer
- optical sectioning" cannot be used as a page name in this wiki.
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software
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ZEN Blue 2012
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incubation
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Not available
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links
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- [ manual]
- [ laser power values]
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- [ beam path]
- [ advanced system check]
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inv.nr.
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0
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