W2 - DeltaVision Core
From BioDIP
(Difference between revisions)
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|applications=Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy. | |applications=Live cell imaging, time lapse, multi point. Tile scanning large samples. High resolution deconvolution fluorescence microscopy. | ||
|image=MPI-W2.jpg | |image=MPI-W2.jpg | ||
+ | |stand-name=Olympus - IX71 | ||
|stand=Inverted | |stand=Inverted | ||
− | |microscope= | + | |microscope=manual, with Applies Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. |
|obj1=Olympus UPlanSApo 10x 0.4 | |obj1=Olympus UPlanSApo 10x 0.4 | ||
|obj2=Olympus UPlanSApo 20x 0.75 0.65mm WD | |obj2=Olympus UPlanSApo 20x 0.75 0.65mm WD |
Revision as of 17:57, 30 March 2010
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Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, with Applies Precision add on equipment: motorized stage, weather station black env control box, with custom built co2 control. | ||
objectives | |||
illumination | |||
detection | Photometrics Cool Snap HQ2 http://www.photomet.com/products/ccdcams/coolsnap_hq2.php | ||
reflectors | Modern hard coated "ET" filters (apart from DAPI exciter)
Fast filter wheels for fluo exciters and ND filters in front of xenon lamp. D350/50x ET402/15x ET555/35x ET645/30x Fast fluo emitter wheel in front of camera. ET455/50m ET525/36m ET605/52m ET705/72m Manual emitters in front of eyepeices. DAPI, FTIC, TRITC. Polychromatic beam splitters for 1) DAPI, FITC, RD-TR-PE, Cy5 in filter cube position1: T89100bs. and 2) ET dualband GFP/mCherry in position 2. See spectra sheet at system. | ||
features | Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples."Built in deconvolution software and optimised/selected precision optical hardware. AOI scan (hardware z fast sum projection), tile scan, multi point scan, env control. Easy to use software. Much faster for large field of view than single point scanning confocal. Blue Galss / UV filter in excitation path for live cell imaging. ND filters for gentle illumination of living samples." cannot be used as a page name in this wiki. | ||
software | Applied Precision SoftWorx running on a Cent OS Linux box. Hardware controlled indirectly via a windows XP machine also on the system. | ||
incubation | Cage incubator (T+CO2) | ||
links |
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inv.nr. | MOZGO 4076530000 |