SD1 - Andor Spinning Disc
From BioDIP
(Difference between revisions)
(added prior xy scanning stage one change before, now: updated 561 laser power to correct value of 75mW) |
(added info about projecting lens magnification) |
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|building=MPI-CBG | |building=MPI-CBG | ||
|room=038B | |room=038B | ||
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|description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW) + 561 nm solid-state Cobolt Jive (75mW). | |description=Fast, "real-time" confocal imaging. Laser equipment: 488 nm solid-state Coherent Sapphire (75mW) + 561 nm solid-state Cobolt Jive (75mW). | ||
|applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution. | |applications=confocal imaging of EGFP and mCherry with high temporal and spatial resolution. | ||
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|stand-name=Olympus - IX71 | |stand-name=Olympus - IX71 | ||
|stand=Inverted | |stand=Inverted | ||
− | |microscope=manual, 1.6x OptoVar, fast piezo z-drive for objective, Prior ProScanIII xy scanning stage | + | |microscope=manual, 1.6x OptoVar, fast piezo z-drive for objective, Prior ProScanIII xy scanning stage |
|obj1=Olympus UPlanSApo 10x 0.4 | |obj1=Olympus UPlanSApo 10x 0.4 | ||
|obj2=Olympus UPlanSApo 60x 1.20 W | |obj2=Olympus UPlanSApo 60x 1.20 W | ||
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|ill3=Laser DPSS 488 nm | |ill3=Laser DPSS 488 nm | ||
|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
− | |detection=Spinning disc scan head Yokogawa CSU10: dichromatic mirror: [http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=855&CategoryID=82 T-405/488/568/647]<br> physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um | + | |detection=Spinning disc scan head Yokogawa CSU10: dichromatic mirror: [http://www.semrock.com/Catalog/Detail.aspx?FilterPartID=855&CategoryID=82 T-405/488/568/647]<br> physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar)<br>Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um<br>image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um<br>camera projecting lens has about 0.9x magnification calculated from the measured pixel sizes |
|reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: 716/40<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | |reflectors=Optical Insight DualView image splitter: <br>HC 520/35; BS 565; HC 628/40<br>Sutter filter wheel: <br>position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: 716/40<br> microscope filter turret:<br> position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
|features=- | |features=- | ||
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|link2=- | |link2=- | ||
|inv=0 | |inv=0 | ||
+ | |facility_image=logo-lmf.jpg | ||
|objectives=10x / 0.30 Plan-Neofluar<br>60x / 1.20 W UPlanSApo<br>100x / 1.40 Oil UPlanSApo | |objectives=10x / 0.30 Plan-Neofluar<br>60x / 1.20 W UPlanSApo<br>100x / 1.40 Oil UPlanSApo | ||
|illumination=Halogen<br>HBO<br>488 nm solid-state laser Coherent Sapphire, 75mW<br>561 nm solid-state laser Cobolt Jive, 40mW | |illumination=Halogen<br>HBO<br>488 nm solid-state laser Coherent Sapphire, 75mW<br>561 nm solid-state laser Cobolt Jive, 40mW | ||
}} | }} |
Revision as of 14:13, 28 February 2011
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Olympus - IX71, Inverted stand, manual, 1.6x OptoVar, fast piezo z-drive for objective, Prior ProScanIII xy scanning stage | ||
objectives | |||
illumination | |||
detection | [[detection::Spinning disc scan head Yokogawa CSU10: dichromatic mirror: T-405/488/568/647 physical pinhole radius: 24um, physical pinhole spacing: 240um; back-projected pinhole radius: 0.15um, back-projected pinhole spacing: 1.5um (with 100x objective, 1.6x optovar) Andor iXon EM+ DU-897 BV back illuminated EMCCD; pixel size of EMCCD chip: 16um image pixel size (measured): with 100x objective: 0.178um; 100x obj. and 1.6x optovar: 0.111um; 60x obj.: 0.288um; 60x obj. and 1.6x opt.: 0.181um camera projecting lens has about 0.9x magnification calculated from the measured pixel sizes]] | ||
reflectors | Optical Insight DualView image splitter: HC 520/35; BS 565; HC 628/40 Sutter filter wheel: position 0: 624/40, 1: open, 2: 525/30, 3: razor edge 568LP, 4: dual emitter: 512/23+630/91, 5: razor edge 488LP, 6: old CSU dual emitter: 525/30 + 650/?, 7: 605/70, 8: 445/40, 9: 716/40 microscope filter turret: position 1: GFP cube, 2: RFP cube, 3: dual GFP + RFP cube, 4: DAPI cube, 5: empty, 6: DIC analyzer | ||
features | - | ||
software | iQ 1.9.1 | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | 0 |