H1 - DeltaVision OMX - pDV
From BioDIP
(Difference between revisions)
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|room=036 | |room=036 | ||
|Facility-logo=MPI-CBG LMF.jpg | |Facility-logo=MPI-CBG LMF.jpg | ||
− | |description=Structured Illumination Superresolution and fast widefield. 4x | + | |description=Structured Illumination Superresolution and very fast widefield. 4x sCMOS, 4x laser illumination widefield or structured 3D illumination. Also has its own personal DeltaVision widefield fluorescence microscope system with a stage that is registered with the OMX stage, for locating samples on the slide. |
− | |applications=super resolution down to 120 nm, and fast widefield imaging. | + | |applications=super resolution of fixed and living samples down to 120 nm, and very fast widefield imaging. |
|image=H1-OMX.jpg | |image=H1-OMX.jpg | ||
|stand-name=Applied Precision - OMX stand | |stand-name=Applied Precision - OMX stand | ||
|stand=Inverted | |stand=Inverted | ||
− | |microscope=nano precise XYZ stage, fluorescence, transmitted light, DIC, | + | |microscope=nano precise XYZ stage, fast z peizo, , fast structured illumination OMX Blaze, fluorescence, transmitted light, DIC, plus IX71 for the pDV. |
− | plus IX71 for the pDV. | + | |
|obj1=Olympus UPlanSApo 100x 1.4 Oil | |obj1=Olympus UPlanSApo 100x 1.4 Oil | ||
|obj2=Olympus UPlanSApo 10x 0.4 | |obj2=Olympus UPlanSApo 10x 0.4 | ||
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|ill4=Laser DPSS 561 nm | |ill4=Laser DPSS 561 nm | ||
|ill5=Laser DPSS 640 nm | |ill5=Laser DPSS 640 nm | ||
− | |detection=4x | + | |detection=4x sCMOS cooled to 3oC. Simultaneous or sequential imaging. |
− | |reflectors=standard set, OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set. | + | |reflectors="standard" set for fixed samples (also GFP, and some red proteins), OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set. |
− | |features=fast 4 colour widefield. Super resolution by structured illumination in 3D, down to | + | |features=fast 4 colour widefield. Super resolution by structured illumination in 3D, down to approx 120 nm resolution (2x the resolution of conventional light microscopy) |
|software=API softworx and OMX software for driving the scope and super resolution reconstruction. | |software=API softworx and OMX software for driving the scope and super resolution reconstruction. | ||
OMX SI reconstruction linux box: centos 4, 10.1.145.31 | OMX SI reconstruction linux box: centos 4, 10.1.145.31 |
Revision as of 12:19, 11 January 2012
Directions
[[image:|150px|text-bottom|right]]
Booking
https://python-srv1.mpi-cbg.de/lmf-ipf/cgi-bin/index.py
Details
microscope | Applied Precision - OMX stand, Inverted stand, nano precise XYZ stage, fast z peizo, , fast structured illumination OMX Blaze, fluorescence, transmitted light, DIC, plus IX71 for the pDV. | ||
objectives | |||
illumination | |||
detection | 4x sCMOS cooled to 3oC. Simultaneous or sequential imaging. | ||
reflectors | "standard" set for fixed samples (also GFP, and some red proteins), OMX. See Docs at OMX. DAPI/FITC/TRITC/Cy5 - similar to Sedat Set. | ||
features | fast 4 colour widefield. Super resolution by structured illumination in 3D, down to approx 120 nm resolution (2x the resolution of conventional light microscopy) | ||
software | API softworx and OMX software for driving the scope and super resolution reconstruction.
OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32"API softworx and OMX software for driving the scope and super resolution reconstruction. OMX SI reconstruction linux box: centos 4, 10.1.145.31 personalDV linux centos5 box 10.1.145.32" cannot be used as a page name in this wiki. | ||
incubation | Stage incubator (T) + objective heater | ||
links |
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inv.nr. | MOZG040809900 |